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Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1

Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a significant association between anti-laminin-1 antibodies (Abs) and reproductive failure, such as rec...

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Autores principales: Kondo, Akane, Inagaki, Junko, Kobayashi, Kazuko, Tsukamoto, Hideo, Yamamoto, Daisuke, Nakatsuka, Mikiya, Suzuki, Nobuharu, Nomizu, Motoyoshi, Amano, Satoshi, Matsubayashi, Hidehiko, Yasuda, Tatsuji, Lopez, Luis R., Shoenfeld, Yehuda, Makino, Tsunehisa, Matsuura, Eiji
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2270721/
https://www.ncbi.nlm.nih.gov/pubmed/15712601
http://dx.doi.org/10.1080/17402520400014168
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author Kondo, Akane
Inagaki, Junko
Kobayashi, Kazuko
Tsukamoto, Hideo
Yamamoto, Daisuke
Nakatsuka, Mikiya
Suzuki, Nobuharu
Nomizu, Motoyoshi
Amano, Satoshi
Matsubayashi, Hidehiko
Yasuda, Tatsuji
Lopez, Luis R.
Shoenfeld, Yehuda
Makino, Tsunehisa
Matsuura, Eiji
author_facet Kondo, Akane
Inagaki, Junko
Kobayashi, Kazuko
Tsukamoto, Hideo
Yamamoto, Daisuke
Nakatsuka, Mikiya
Suzuki, Nobuharu
Nomizu, Motoyoshi
Amano, Satoshi
Matsubayashi, Hidehiko
Yasuda, Tatsuji
Lopez, Luis R.
Shoenfeld, Yehuda
Makino, Tsunehisa
Matsuura, Eiji
author_sort Kondo, Akane
collection PubMed
description Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a significant association between anti-laminin-1 antibodies (Abs) and reproductive failure, such as recurrent spontaneous abortions and infertility-associated endometriosis in both human and mouse studies. In the present study, we established an IgM (μ,κ) monoclonal anti-laminin-1 Ab (AK8) by immunizing mice with mouse Engelbreth-Holm-Swarm sarcoma (EHS)-derived laminin-α1. The AK8 monoclonal antibody (mAb) reacted with particular peptide sequences from the globular G domain of mouse laminin-α1 chain of using ELISA and Western blot techniques. The peptide tertiary structure of the epitope recognized by AK8 mAb was predicted using eight synthesized domain peptide sequences and three consensus sequences obtained by phage displayed random peptide library. Basement membranes of endometrium of pregnant mice and humans were immunostained with AK8 mAb. Thus, AK8 mAb recognized a common structure present in the G domain of the laminin-1 chain in both mice and humans. The passive immunization of mice with AK8 mAb may represent a suitable animal model for anti-laminin-1 Ab-mediated reproductive failure.
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spelling pubmed-22707212008-03-31 Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1 Kondo, Akane Inagaki, Junko Kobayashi, Kazuko Tsukamoto, Hideo Yamamoto, Daisuke Nakatsuka, Mikiya Suzuki, Nobuharu Nomizu, Motoyoshi Amano, Satoshi Matsubayashi, Hidehiko Yasuda, Tatsuji Lopez, Luis R. Shoenfeld, Yehuda Makino, Tsunehisa Matsuura, Eiji Clin Dev Immunol Research Article Laminin-1 is a structural glycoprotein that forms an integral part of the scaffolding of basement membranes, and plays an important role during embryonic development. We have recently demonstrated a significant association between anti-laminin-1 antibodies (Abs) and reproductive failure, such as recurrent spontaneous abortions and infertility-associated endometriosis in both human and mouse studies. In the present study, we established an IgM (μ,κ) monoclonal anti-laminin-1 Ab (AK8) by immunizing mice with mouse Engelbreth-Holm-Swarm sarcoma (EHS)-derived laminin-α1. The AK8 monoclonal antibody (mAb) reacted with particular peptide sequences from the globular G domain of mouse laminin-α1 chain of using ELISA and Western blot techniques. The peptide tertiary structure of the epitope recognized by AK8 mAb was predicted using eight synthesized domain peptide sequences and three consensus sequences obtained by phage displayed random peptide library. Basement membranes of endometrium of pregnant mice and humans were immunostained with AK8 mAb. Thus, AK8 mAb recognized a common structure present in the G domain of the laminin-1 chain in both mice and humans. The passive immunization of mice with AK8 mAb may represent a suitable animal model for anti-laminin-1 Ab-mediated reproductive failure. Hindawi Publishing Corporation 2005-03 /pmc/articles/PMC2270721/ /pubmed/15712601 http://dx.doi.org/10.1080/17402520400014168 Text en Copyright © 2005 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kondo, Akane
Inagaki, Junko
Kobayashi, Kazuko
Tsukamoto, Hideo
Yamamoto, Daisuke
Nakatsuka, Mikiya
Suzuki, Nobuharu
Nomizu, Motoyoshi
Amano, Satoshi
Matsubayashi, Hidehiko
Yasuda, Tatsuji
Lopez, Luis R.
Shoenfeld, Yehuda
Makino, Tsunehisa
Matsuura, Eiji
Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
title Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
title_full Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
title_fullStr Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
title_full_unstemmed Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
title_short Characterization of a Murine Anti-laminin-1 Monoclonal Antibody (AK8) Produced by Immunization with Mouse-derived Laminin-1
title_sort characterization of a murine anti-laminin-1 monoclonal antibody (ak8) produced by immunization with mouse-derived laminin-1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2270721/
https://www.ncbi.nlm.nih.gov/pubmed/15712601
http://dx.doi.org/10.1080/17402520400014168
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