FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration

Cell migration involves a multitude of signals that converge on cytoskeletal reorganization, essential for development, immune responses and tissue repair. Using knockdown and dominant negative approaches, we show that the microtubule-associated Ste20-like kinase SLK is required for focal adhesion t...

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Autores principales: Wagner, Simona, Storbeck, Chris J., Roovers, Kristin, Chaar, Ziad Y., Kolodziej, Piotr, McKay, Marlene, Sabourin, Luc A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2270904/
https://www.ncbi.nlm.nih.gov/pubmed/18382658
http://dx.doi.org/10.1371/journal.pone.0001868
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author Wagner, Simona
Storbeck, Chris J.
Roovers, Kristin
Chaar, Ziad Y.
Kolodziej, Piotr
McKay, Marlene
Sabourin, Luc A.
author_facet Wagner, Simona
Storbeck, Chris J.
Roovers, Kristin
Chaar, Ziad Y.
Kolodziej, Piotr
McKay, Marlene
Sabourin, Luc A.
author_sort Wagner, Simona
collection PubMed
description Cell migration involves a multitude of signals that converge on cytoskeletal reorganization, essential for development, immune responses and tissue repair. Using knockdown and dominant negative approaches, we show that the microtubule-associated Ste20-like kinase SLK is required for focal adhesion turnover and cell migration downstream of the FAK/c-src complex. Our results show that SLK co-localizes with paxillin, Rac1 and the microtubules at the leading edge of migrating cells and is activated by scratch wounding. SLK activation is dependent on FAK/c-src/MAPK signaling, whereas SLK recruitment to the leading edge is src-dependent but FAK independent. Our results show that SLK represents a novel focal adhesion disassembly signal.
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spelling pubmed-22709042008-04-02 FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration Wagner, Simona Storbeck, Chris J. Roovers, Kristin Chaar, Ziad Y. Kolodziej, Piotr McKay, Marlene Sabourin, Luc A. PLoS One Research Article Cell migration involves a multitude of signals that converge on cytoskeletal reorganization, essential for development, immune responses and tissue repair. Using knockdown and dominant negative approaches, we show that the microtubule-associated Ste20-like kinase SLK is required for focal adhesion turnover and cell migration downstream of the FAK/c-src complex. Our results show that SLK co-localizes with paxillin, Rac1 and the microtubules at the leading edge of migrating cells and is activated by scratch wounding. SLK activation is dependent on FAK/c-src/MAPK signaling, whereas SLK recruitment to the leading edge is src-dependent but FAK independent. Our results show that SLK represents a novel focal adhesion disassembly signal. Public Library of Science 2008-04-02 /pmc/articles/PMC2270904/ /pubmed/18382658 http://dx.doi.org/10.1371/journal.pone.0001868 Text en Wagner et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wagner, Simona
Storbeck, Chris J.
Roovers, Kristin
Chaar, Ziad Y.
Kolodziej, Piotr
McKay, Marlene
Sabourin, Luc A.
FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration
title FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration
title_full FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration
title_fullStr FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration
title_full_unstemmed FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration
title_short FAK/src-Family Dependent Activation of the Ste20-Like Kinase SLK Is Required for Microtubule-Dependent Focal Adhesion Turnover and Cell Migration
title_sort fak/src-family dependent activation of the ste20-like kinase slk is required for microtubule-dependent focal adhesion turnover and cell migration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2270904/
https://www.ncbi.nlm.nih.gov/pubmed/18382658
http://dx.doi.org/10.1371/journal.pone.0001868
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