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The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry

Two to three percent of the world's population is chronically infected with hepatitis C virus (HCV) and thus at risk of developing liver cancer. Although precise mechanisms regulating HCV entry into hepatic cells are still unknown, several cell surface proteins have been identified as entry fac...

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Autores principales: Rocha-Perugini, Vera, Montpellier, Claire, Delgrange, David, Wychowski, Czeslaw, Helle, François, Pillez, André, Drobecq, Hervé, Le Naour, François, Charrin, Stéphanie, Levy, Shoshana, Rubinstein, Eric, Dubuisson, Jean, Cocquerel, Laurence
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2270906/
https://www.ncbi.nlm.nih.gov/pubmed/18382656
http://dx.doi.org/10.1371/journal.pone.0001866
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author Rocha-Perugini, Vera
Montpellier, Claire
Delgrange, David
Wychowski, Czeslaw
Helle, François
Pillez, André
Drobecq, Hervé
Le Naour, François
Charrin, Stéphanie
Levy, Shoshana
Rubinstein, Eric
Dubuisson, Jean
Cocquerel, Laurence
author_facet Rocha-Perugini, Vera
Montpellier, Claire
Delgrange, David
Wychowski, Czeslaw
Helle, François
Pillez, André
Drobecq, Hervé
Le Naour, François
Charrin, Stéphanie
Levy, Shoshana
Rubinstein, Eric
Dubuisson, Jean
Cocquerel, Laurence
author_sort Rocha-Perugini, Vera
collection PubMed
description Two to three percent of the world's population is chronically infected with hepatitis C virus (HCV) and thus at risk of developing liver cancer. Although precise mechanisms regulating HCV entry into hepatic cells are still unknown, several cell surface proteins have been identified as entry factors for this virus. Among these molecules, the tetraspanin CD81 is essential for HCV entry. Here, we have identified a partner of CD81, EWI-2wint, which is expressed in several cell lines but not in hepatocytes. Ectopic expression of EWI-2wint in a hepatoma cell line susceptible to HCV infection blocked viral entry by inhibiting the interaction between the HCV envelope glycoproteins and CD81. This finding suggests that, in addition to the presence of specific entry factors in the hepatocytes, the lack of a specific inhibitor can contribute to the hepatotropism of HCV. This is the first example of a pathogen gaining entry into host cells that lack a specific inhibitory factor.
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spelling pubmed-22709062008-04-02 The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry Rocha-Perugini, Vera Montpellier, Claire Delgrange, David Wychowski, Czeslaw Helle, François Pillez, André Drobecq, Hervé Le Naour, François Charrin, Stéphanie Levy, Shoshana Rubinstein, Eric Dubuisson, Jean Cocquerel, Laurence PLoS One Research Article Two to three percent of the world's population is chronically infected with hepatitis C virus (HCV) and thus at risk of developing liver cancer. Although precise mechanisms regulating HCV entry into hepatic cells are still unknown, several cell surface proteins have been identified as entry factors for this virus. Among these molecules, the tetraspanin CD81 is essential for HCV entry. Here, we have identified a partner of CD81, EWI-2wint, which is expressed in several cell lines but not in hepatocytes. Ectopic expression of EWI-2wint in a hepatoma cell line susceptible to HCV infection blocked viral entry by inhibiting the interaction between the HCV envelope glycoproteins and CD81. This finding suggests that, in addition to the presence of specific entry factors in the hepatocytes, the lack of a specific inhibitor can contribute to the hepatotropism of HCV. This is the first example of a pathogen gaining entry into host cells that lack a specific inhibitory factor. Public Library of Science 2008-04-02 /pmc/articles/PMC2270906/ /pubmed/18382656 http://dx.doi.org/10.1371/journal.pone.0001866 Text en Rocha-Perugini et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rocha-Perugini, Vera
Montpellier, Claire
Delgrange, David
Wychowski, Czeslaw
Helle, François
Pillez, André
Drobecq, Hervé
Le Naour, François
Charrin, Stéphanie
Levy, Shoshana
Rubinstein, Eric
Dubuisson, Jean
Cocquerel, Laurence
The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
title The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
title_full The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
title_fullStr The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
title_full_unstemmed The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
title_short The CD81 Partner EWI-2wint Inhibits Hepatitis C Virus Entry
title_sort cd81 partner ewi-2wint inhibits hepatitis c virus entry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2270906/
https://www.ncbi.nlm.nih.gov/pubmed/18382656
http://dx.doi.org/10.1371/journal.pone.0001866
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