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Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans
BACKGROUND: Previous work has demonstrated the potential for peripheral blood (PB) gene expression profiling for the detection of disease or environmental exposures. METHODS AND FINDINGS: We have sought to determine the impact of several variables on the PB gene expression profile of an environmenta...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2271127/ https://www.ncbi.nlm.nih.gov/pubmed/18382685 http://dx.doi.org/10.1371/journal.pone.0001912 |
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author | Meadows, Sarah K. Dressman, Holly K. Muramoto, Garrett G. Himburg, Heather Salter, Alice Wei, ZhengZheng Ginsburg, Geoff Chao, Nelson J. Nevins, Joseph R. Chute, John P. |
author_facet | Meadows, Sarah K. Dressman, Holly K. Muramoto, Garrett G. Himburg, Heather Salter, Alice Wei, ZhengZheng Ginsburg, Geoff Chao, Nelson J. Nevins, Joseph R. Chute, John P. |
author_sort | Meadows, Sarah K. |
collection | PubMed |
description | BACKGROUND: Previous work has demonstrated the potential for peripheral blood (PB) gene expression profiling for the detection of disease or environmental exposures. METHODS AND FINDINGS: We have sought to determine the impact of several variables on the PB gene expression profile of an environmental exposure, ionizing radiation, and to determine the specificity of the PB signature of radiation versus other genotoxic stresses. Neither genotype differences nor the time of PB sampling caused any lessening of the accuracy of PB signatures to predict radiation exposure, but sex difference did influence the accuracy of the prediction of radiation exposure at the lowest level (50 cGy). A PB signature of sepsis was also generated and both the PB signature of radiation and the PB signature of sepsis were found to be 100% specific at distinguishing irradiated from septic animals. We also identified human PB signatures of radiation exposure and chemotherapy treatment which distinguished irradiated patients and chemotherapy-treated individuals within a heterogeneous population with accuracies of 90% and 81%, respectively. CONCLUSIONS: We conclude that PB gene expression profiles can be identified in mice and humans that are accurate in predicting medical conditions, are specific to each condition and remain highly accurate over time. |
format | Text |
id | pubmed-2271127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-22711272008-04-02 Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans Meadows, Sarah K. Dressman, Holly K. Muramoto, Garrett G. Himburg, Heather Salter, Alice Wei, ZhengZheng Ginsburg, Geoff Chao, Nelson J. Nevins, Joseph R. Chute, John P. PLoS One Research Article BACKGROUND: Previous work has demonstrated the potential for peripheral blood (PB) gene expression profiling for the detection of disease or environmental exposures. METHODS AND FINDINGS: We have sought to determine the impact of several variables on the PB gene expression profile of an environmental exposure, ionizing radiation, and to determine the specificity of the PB signature of radiation versus other genotoxic stresses. Neither genotype differences nor the time of PB sampling caused any lessening of the accuracy of PB signatures to predict radiation exposure, but sex difference did influence the accuracy of the prediction of radiation exposure at the lowest level (50 cGy). A PB signature of sepsis was also generated and both the PB signature of radiation and the PB signature of sepsis were found to be 100% specific at distinguishing irradiated from septic animals. We also identified human PB signatures of radiation exposure and chemotherapy treatment which distinguished irradiated patients and chemotherapy-treated individuals within a heterogeneous population with accuracies of 90% and 81%, respectively. CONCLUSIONS: We conclude that PB gene expression profiles can be identified in mice and humans that are accurate in predicting medical conditions, are specific to each condition and remain highly accurate over time. Public Library of Science 2008-04-02 /pmc/articles/PMC2271127/ /pubmed/18382685 http://dx.doi.org/10.1371/journal.pone.0001912 Text en Meadows et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Meadows, Sarah K. Dressman, Holly K. Muramoto, Garrett G. Himburg, Heather Salter, Alice Wei, ZhengZheng Ginsburg, Geoff Chao, Nelson J. Nevins, Joseph R. Chute, John P. Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans |
title | Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans |
title_full | Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans |
title_fullStr | Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans |
title_full_unstemmed | Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans |
title_short | Gene Expression Signatures of Radiation Response Are Specific, Durable and Accurate in Mice and Humans |
title_sort | gene expression signatures of radiation response are specific, durable and accurate in mice and humans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2271127/ https://www.ncbi.nlm.nih.gov/pubmed/18382685 http://dx.doi.org/10.1371/journal.pone.0001912 |
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