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Improving image analysis in 2DGE-based redox proteomics by labeling protein carbonyl with fluorescent hydroxylamine

Recent advances in redox proteomics have provided significant insight into the role of oxidative modifications in cellular signalling and metabolism. At present, these techniques rely heavily on Western blots to visualize the oxidative modification and corresponding two dimensional (2D) gels for det...

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Detalles Bibliográficos
Autores principales: Poon, H. Fai, Abdullah, Laila, Reed, Jon, Doore, Sarah M, Laird, Cyndi, Mathura, Venkat, Mullan, Michael, Crawford, Fiona
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2274965/
https://www.ncbi.nlm.nih.gov/pubmed/18385803
http://dx.doi.org/10.1251/bpo134