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Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells

RNA-dependent RNA polymerase 6 (RDR6) catalyses dsRNA synthesis for post-transcriptional gene silencing (PTGS)-associated amplification and the generation of endogeneous siRNAs involved in developmental determinations or stress responses. The functional importance of RDR6 in PTGS led us to examine i...

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Autores principales: Yang, Ji Hyun, Seo, Hyun Hee, Han, So Jung, Yoon, Eun Kyung, Yang, Moon Sik, Lee, Woo Sung
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275079/
https://www.ncbi.nlm.nih.gov/pubmed/18160413
http://dx.doi.org/10.1093/nar/gkm1133
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author Yang, Ji Hyun
Seo, Hyun Hee
Han, So Jung
Yoon, Eun Kyung
Yang, Moon Sik
Lee, Woo Sung
author_facet Yang, Ji Hyun
Seo, Hyun Hee
Han, So Jung
Yoon, Eun Kyung
Yang, Moon Sik
Lee, Woo Sung
author_sort Yang, Ji Hyun
collection PubMed
description RNA-dependent RNA polymerase 6 (RDR6) catalyses dsRNA synthesis for post-transcriptional gene silencing (PTGS)-associated amplification and the generation of endogeneous siRNAs involved in developmental determinations or stress responses. The functional importance of RDR6 in PTGS led us to examine its connection to the cellular regulatory network by analyzing the hormonal responses of RDR6 gene expression in a cultured cell system. Delivery of dsRNA, prepared in vitro, into cultured rice (Oryza sativa cv. Japonica Dongjin) cells successfully silenced the target isocitrate lyase (ICL) transcripts. Silencing was transient in the absence of abscisic acid (ABA), while it became persistent in the presence of ABA in growth medium. A transcription assay of the OsRDR6 promoter showed that it was positively regulated by ABA. OsRDR6-dependent siRNA(ICL) generation was also significantly up-regulated by ABA. The results showed that, among the five rice OsRDR isogenes, only OsRDR6 was responsible for the observed ABA-mediated amplification and silencing of ICL transcripts. We propose that ABA modulates PTGS through the transcriptional control of the OsRDR6 gene.
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spelling pubmed-22750792008-04-07 Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells Yang, Ji Hyun Seo, Hyun Hee Han, So Jung Yoon, Eun Kyung Yang, Moon Sik Lee, Woo Sung Nucleic Acids Res RNA RNA-dependent RNA polymerase 6 (RDR6) catalyses dsRNA synthesis for post-transcriptional gene silencing (PTGS)-associated amplification and the generation of endogeneous siRNAs involved in developmental determinations or stress responses. The functional importance of RDR6 in PTGS led us to examine its connection to the cellular regulatory network by analyzing the hormonal responses of RDR6 gene expression in a cultured cell system. Delivery of dsRNA, prepared in vitro, into cultured rice (Oryza sativa cv. Japonica Dongjin) cells successfully silenced the target isocitrate lyase (ICL) transcripts. Silencing was transient in the absence of abscisic acid (ABA), while it became persistent in the presence of ABA in growth medium. A transcription assay of the OsRDR6 promoter showed that it was positively regulated by ABA. OsRDR6-dependent siRNA(ICL) generation was also significantly up-regulated by ABA. The results showed that, among the five rice OsRDR isogenes, only OsRDR6 was responsible for the observed ABA-mediated amplification and silencing of ICL transcripts. We propose that ABA modulates PTGS through the transcriptional control of the OsRDR6 gene. Oxford University Press 2008-03 2007-12-26 /pmc/articles/PMC2275079/ /pubmed/18160413 http://dx.doi.org/10.1093/nar/gkm1133 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Yang, Ji Hyun
Seo, Hyun Hee
Han, So Jung
Yoon, Eun Kyung
Yang, Moon Sik
Lee, Woo Sung
Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells
title Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells
title_full Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells
title_fullStr Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells
title_full_unstemmed Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells
title_short Phytohormone abscisic acid control RNA-dependent RNA polymerase 6 gene expression and post-transcriptional gene silencing in rice cells
title_sort phytohormone abscisic acid control rna-dependent rna polymerase 6 gene expression and post-transcriptional gene silencing in rice cells
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275079/
https://www.ncbi.nlm.nih.gov/pubmed/18160413
http://dx.doi.org/10.1093/nar/gkm1133
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