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Improved genome-wide localization by ChIP-chip using double-round T7 RNA polymerase-based amplification

Chromatin immunoprecipitation combined with DNA microarrays (ChIP-chip) is a powerful technique to detect in vivo protein–DNA interactions. Due to low yields, ChIP assays of transcription factors generally require amplification of immunoprecipitated genomic DNA. Here, we present an adapted linear am...

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Detalles Bibliográficos
Autores principales: van Bakel, Harm, van Werven, Folkert J., Radonjic, Marijana, Brok, Mariel O., van Leenen, Dik, Holstege, Frank C. P., Timmers, H. T. Marc
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275083/
https://www.ncbi.nlm.nih.gov/pubmed/18180247
http://dx.doi.org/10.1093/nar/gkm1144

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