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Improved genome-wide localization by ChIP-chip using double-round T7 RNA polymerase-based amplification
Chromatin immunoprecipitation combined with DNA microarrays (ChIP-chip) is a powerful technique to detect in vivo protein–DNA interactions. Due to low yields, ChIP assays of transcription factors generally require amplification of immunoprecipitated genomic DNA. Here, we present an adapted linear am...
Autores principales: | van Bakel, Harm, van Werven, Folkert J., Radonjic, Marijana, Brok, Mariel O., van Leenen, Dik, Holstege, Frank C. P., Timmers, H. T. Marc |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275083/ https://www.ncbi.nlm.nih.gov/pubmed/18180247 http://dx.doi.org/10.1093/nar/gkm1144 |
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