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Detection of membrane cholesterol by filipin in isolated rat liver coated vesicles is dependent upon removal of the clathrin coat
We investigated the cholesterol content of highly purified populations of coated vesicles from rat liver by biochemical quantitation and by cytochemical electron microscopy using the polyene antibiotic filipin. Failure of this reagent to elicit its typical response for a cholesterol-containing membr...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1984
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275625/ https://www.ncbi.nlm.nih.gov/pubmed/6145719 |