Cargando…
Actin in the photoreceptor connecting cilium: immunocytochemical localization to the site of outer segment disk formation
Actin has been localized in Rana pipiens retinas that were fixed and embedded in aldehyde cross-linked BSA. Thin sections were reacted sequentially with (a) affinity-purified antiactin antibodies induced in rabbits; (b) biotinyl-sheep anti-rabbit antibodies; and (c) avidin- ferritin conjugates. As e...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1984
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275634/ https://www.ncbi.nlm.nih.gov/pubmed/6610682 |
_version_ | 1782151888716693504 |
---|---|
collection | PubMed |
description | Actin has been localized in Rana pipiens retinas that were fixed and embedded in aldehyde cross-linked BSA. Thin sections were reacted sequentially with (a) affinity-purified antiactin antibodies induced in rabbits; (b) biotinyl-sheep anti-rabbit antibodies; and (c) avidin- ferritin conjugates. As expected, antiactin labeling density was high in the apical pigment epithelial cell processes and in the calycal processes of photoreceptors. Actin was also localized in a new site. The connecting cilium that joins the inner and outer segments of both rods and cones was heavily labeled by antiactin at its outer segment (OS), or distal, end. In this region of the cilium, the plasma membrane evaginates to form new OS disks and these basal disks were labeled in some instances. Below the new disks in rods, the cytoplasm of liplike expansions of the distal cilium was also heavily labeled. The plasma membrane and interior of the connecting cilium and the remainder of the OS were unlabeled. These findings suggest that actin may participate in the vectorial transport of opsin and other intrinsic membrane proteins that are incorporated into newly forming OS disks. The results also implicate actin in the membrane expansion involved with OS disk formation. |
format | Text |
id | pubmed-2275634 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1984 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22756342008-05-01 Actin in the photoreceptor connecting cilium: immunocytochemical localization to the site of outer segment disk formation J Cell Biol Articles Actin has been localized in Rana pipiens retinas that were fixed and embedded in aldehyde cross-linked BSA. Thin sections were reacted sequentially with (a) affinity-purified antiactin antibodies induced in rabbits; (b) biotinyl-sheep anti-rabbit antibodies; and (c) avidin- ferritin conjugates. As expected, antiactin labeling density was high in the apical pigment epithelial cell processes and in the calycal processes of photoreceptors. Actin was also localized in a new site. The connecting cilium that joins the inner and outer segments of both rods and cones was heavily labeled by antiactin at its outer segment (OS), or distal, end. In this region of the cilium, the plasma membrane evaginates to form new OS disks and these basal disks were labeled in some instances. Below the new disks in rods, the cytoplasm of liplike expansions of the distal cilium was also heavily labeled. The plasma membrane and interior of the connecting cilium and the remainder of the OS were unlabeled. These findings suggest that actin may participate in the vectorial transport of opsin and other intrinsic membrane proteins that are incorporated into newly forming OS disks. The results also implicate actin in the membrane expansion involved with OS disk formation. The Rockefeller University Press 1984-07-01 /pmc/articles/PMC2275634/ /pubmed/6610682 Text en Copyright © 1984, This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Actin in the photoreceptor connecting cilium: immunocytochemical localization to the site of outer segment disk formation |
title | Actin in the photoreceptor connecting cilium: immunocytochemical
localization to the site of outer segment disk formation |
title_full | Actin in the photoreceptor connecting cilium: immunocytochemical
localization to the site of outer segment disk formation |
title_fullStr | Actin in the photoreceptor connecting cilium: immunocytochemical
localization to the site of outer segment disk formation |
title_full_unstemmed | Actin in the photoreceptor connecting cilium: immunocytochemical
localization to the site of outer segment disk formation |
title_short | Actin in the photoreceptor connecting cilium: immunocytochemical
localization to the site of outer segment disk formation |
title_sort | actin in the photoreceptor connecting cilium: immunocytochemical
localization to the site of outer segment disk formation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275634/ https://www.ncbi.nlm.nih.gov/pubmed/6610682 |