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Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum

The temporal relationship between tubulin expression and the assembly of the mitotic spindle microtubules has been investigated during the naturally synchronous cell cycle of the Physarum plasmodium. The cell cycle behavior of the tubulin isoforms was examined by two-dimensional gel electrophoresis...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275639/
https://www.ncbi.nlm.nih.gov/pubmed/6203916
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collection PubMed
description The temporal relationship between tubulin expression and the assembly of the mitotic spindle microtubules has been investigated during the naturally synchronous cell cycle of the Physarum plasmodium. The cell cycle behavior of the tubulin isoforms was examined by two-dimensional gel electrophoresis of proteins labeled in vivo and by translation of RNA in vitro. alpha 1-, alpha 2-, beta 1-, and beta 2-tubulin synthesis increases coordinately until metaphase, and then falls, with beta 2 falling more rapidly than beta 1. Nucleic acid hybridization demonstrated that alpha- and beta-tubulin RNAs accumulate coordinately during G2, peaking at metaphase. Quantitative analysis demonstrated that alpha-tubulin RNA increases with apparent exponential kinetics, peaking with an increase over the basal level of greater than 40-fold. After metaphase, tubulin RNA levels fall exponentially, with a short half-life (19 min). Electron microscopic analysis of the plasmodium showed that the accumulation of tubulin RNA begins long before the polymerization of mitotic spindle microtubules. By contrast, the decay of tubulin RNA after metaphase coincides with the depolymerization of the spindle microtubules.
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spelling pubmed-22756392008-05-01 Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum J Cell Biol Articles The temporal relationship between tubulin expression and the assembly of the mitotic spindle microtubules has been investigated during the naturally synchronous cell cycle of the Physarum plasmodium. The cell cycle behavior of the tubulin isoforms was examined by two-dimensional gel electrophoresis of proteins labeled in vivo and by translation of RNA in vitro. alpha 1-, alpha 2-, beta 1-, and beta 2-tubulin synthesis increases coordinately until metaphase, and then falls, with beta 2 falling more rapidly than beta 1. Nucleic acid hybridization demonstrated that alpha- and beta-tubulin RNAs accumulate coordinately during G2, peaking at metaphase. Quantitative analysis demonstrated that alpha-tubulin RNA increases with apparent exponential kinetics, peaking with an increase over the basal level of greater than 40-fold. After metaphase, tubulin RNA levels fall exponentially, with a short half-life (19 min). Electron microscopic analysis of the plasmodium showed that the accumulation of tubulin RNA begins long before the polymerization of mitotic spindle microtubules. By contrast, the decay of tubulin RNA after metaphase coincides with the depolymerization of the spindle microtubules. The Rockefeller University Press 1984-07-01 /pmc/articles/PMC2275639/ /pubmed/6203916 Text en Copyright © 1984, This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum
title Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum
title_full Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum
title_fullStr Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum
title_full_unstemmed Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum
title_short Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum
title_sort cell cycle regulation of tubulin rna level, tubulin protein synthesis, and assembly of microtubules in physarum
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275639/
https://www.ncbi.nlm.nih.gov/pubmed/6203916