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Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression

We show that vimentin filaments are present in undifferentiated Friend murine erythroleukemia cells, but are lost progressively to undetectable levels by 96 h of dimethyl sulfoxide-mediated differentiation. The amount of newly synthesized cytoskeletal vimentin is decreased dramatically by 24 h of in...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275651/
https://www.ncbi.nlm.nih.gov/pubmed/6376521
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description We show that vimentin filaments are present in undifferentiated Friend murine erythroleukemia cells, but are lost progressively to undetectable levels by 96 h of dimethyl sulfoxide-mediated differentiation. The amount of newly synthesized cytoskeletal vimentin is decreased dramatically by 24 h of induction, and is paralleled by a rapid loss of vimentin mRNA (approximately 25-fold reduction at 96 h). Hence, disappearance of vimentin filaments in these cells appears to be regulated at the level of vimentin mRNA abundance. On the other hand, the levels of actin synthesis and actin mRNA remain essentially unchanged. The kinetics of vimentin mRNA reduction during dimethyl sulfoxide-mediated differentiation, and the levels of vimentin mRNA observed in the presence of hexamethylene-bisacetamide or hemin as inducers suggest that the cessation of vimentin expression precedes, but may be associated with commitment to terminal differentiation. Our results demonstrate the dynamic regulation of vimentin expression in mammalian erythropoiesis.
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spelling pubmed-22756512008-05-01 Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression J Cell Biol Articles We show that vimentin filaments are present in undifferentiated Friend murine erythroleukemia cells, but are lost progressively to undetectable levels by 96 h of dimethyl sulfoxide-mediated differentiation. The amount of newly synthesized cytoskeletal vimentin is decreased dramatically by 24 h of induction, and is paralleled by a rapid loss of vimentin mRNA (approximately 25-fold reduction at 96 h). Hence, disappearance of vimentin filaments in these cells appears to be regulated at the level of vimentin mRNA abundance. On the other hand, the levels of actin synthesis and actin mRNA remain essentially unchanged. The kinetics of vimentin mRNA reduction during dimethyl sulfoxide-mediated differentiation, and the levels of vimentin mRNA observed in the presence of hexamethylene-bisacetamide or hemin as inducers suggest that the cessation of vimentin expression precedes, but may be associated with commitment to terminal differentiation. Our results demonstrate the dynamic regulation of vimentin expression in mammalian erythropoiesis. The Rockefeller University Press 1984-07-01 /pmc/articles/PMC2275651/ /pubmed/6376521 Text en Copyright © 1984, This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression
title Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression
title_full Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression
title_fullStr Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression
title_full_unstemmed Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression
title_short Differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression
title_sort differentiation of murine erythroleukemia cells results in the rapid repression of vimentin gene expression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275651/
https://www.ncbi.nlm.nih.gov/pubmed/6376521