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Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen
BACKGROUND: Pollens from different olive (Olea europaea L.) cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is, in addition, characterized by a high degree of polymorphism in its sequence. The purpose of this study is to...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275730/ https://www.ncbi.nlm.nih.gov/pubmed/18218146 http://dx.doi.org/10.1186/1471-2229-8-10 |
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author | Hamman-Khalifa, AbdelMounim Castro, Antonio Jesús Jiménez-López, José Carlos Rodríguez-García, María Isabel Alché, Juan de Dios |
author_facet | Hamman-Khalifa, AbdelMounim Castro, Antonio Jesús Jiménez-López, José Carlos Rodríguez-García, María Isabel Alché, Juan de Dios |
author_sort | Hamman-Khalifa, AbdelMounim |
collection | PubMed |
description | BACKGROUND: Pollens from different olive (Olea europaea L.) cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is, in addition, characterized by a high degree of polymorphism in its sequence. The purpose of this study is to evaluate the putative presence of divergences in Ole e 1 sequences from different olive cultivars. RESULTS: RNA from pollen individually collected from 10 olive cultivars was used to amplify Ole e 1 sequences by RT-PCR, and the sequences were analyzed by using different bioinformatics tools. Numerous nucleotide substitutions were detected throughout the sequences, many of which resulted in amino acid substitutions in the deduced protein sequences. In most cases variability within a single variety was much lower than among varieties. Key amino acid changes in comparison with "canonical" sequences previously described in the literature included: a) the substitution of C19-relevant to the disulphide bond structure of the protein-, b) the presence of an additional N-glycosylation motif, and c) point substitutions affecting regions of Ole e 1 already described like relevant for the immunogenicity/allergenicity of the protein. CONCLUSION: Varietal origin of olive pollen is a major factor determining the diversity of Ole e 1 variants. We consider this information of capital importance for the optimal design of efficient and safe allergen formulations, and useful for the genetic engineering of modified forms of the allergen among other applications. |
format | Text |
id | pubmed-2275730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22757302008-03-27 Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen Hamman-Khalifa, AbdelMounim Castro, Antonio Jesús Jiménez-López, José Carlos Rodríguez-García, María Isabel Alché, Juan de Dios BMC Plant Biol Research Article BACKGROUND: Pollens from different olive (Olea europaea L.) cultivars have been shown to differ significantly in their content in Ole e 1 and in their overall allergenicity. This allergen is, in addition, characterized by a high degree of polymorphism in its sequence. The purpose of this study is to evaluate the putative presence of divergences in Ole e 1 sequences from different olive cultivars. RESULTS: RNA from pollen individually collected from 10 olive cultivars was used to amplify Ole e 1 sequences by RT-PCR, and the sequences were analyzed by using different bioinformatics tools. Numerous nucleotide substitutions were detected throughout the sequences, many of which resulted in amino acid substitutions in the deduced protein sequences. In most cases variability within a single variety was much lower than among varieties. Key amino acid changes in comparison with "canonical" sequences previously described in the literature included: a) the substitution of C19-relevant to the disulphide bond structure of the protein-, b) the presence of an additional N-glycosylation motif, and c) point substitutions affecting regions of Ole e 1 already described like relevant for the immunogenicity/allergenicity of the protein. CONCLUSION: Varietal origin of olive pollen is a major factor determining the diversity of Ole e 1 variants. We consider this information of capital importance for the optimal design of efficient and safe allergen formulations, and useful for the genetic engineering of modified forms of the allergen among other applications. BioMed Central 2008-01-25 /pmc/articles/PMC2275730/ /pubmed/18218146 http://dx.doi.org/10.1186/1471-2229-8-10 Text en Copyright © 2008 Hamman-Khalifa et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hamman-Khalifa, AbdelMounim Castro, Antonio Jesús Jiménez-López, José Carlos Rodríguez-García, María Isabel Alché, Juan de Dios Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen |
title | Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen |
title_full | Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen |
title_fullStr | Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen |
title_full_unstemmed | Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen |
title_short | Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen |
title_sort | olive cultivar origin is a major cause of polymorphism for ole e 1 pollen allergen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275730/ https://www.ncbi.nlm.nih.gov/pubmed/18218146 http://dx.doi.org/10.1186/1471-2229-8-10 |
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