Cargando…

Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent

Class II major histocompatibility complex (MHC) antigens are expressed predominantly on B lymphocytes and macrophages of tadpoles of the South African clawed frog, Xenopus laevis, as is the pattern in lymphocyte populations of most mammals. However, unlike most mammals, young postmetamorphic frogs s...

Descripción completa

Detalles Bibliográficos
Autores principales: Rollins-Smith, Louise A., Blair, Patrick
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275827/
https://www.ncbi.nlm.nih.gov/pubmed/1967017
http://dx.doi.org/10.1155/1990/25197
_version_ 1782151913102376960
author Rollins-Smith, Louise A.
Blair, Patrick
author_facet Rollins-Smith, Louise A.
Blair, Patrick
author_sort Rollins-Smith, Louise A.
collection PubMed
description Class II major histocompatibility complex (MHC) antigens are expressed predominantly on B lymphocytes and macrophages of tadpoles of the South African clawed frog, Xenopus laevis, as is the pattern in lymphocyte populations of most mammals. However, unlike most mammals, young postmetamorphic frogs show expression of class II MHC antigens on a high proportion of thymocytes and most peripheral T and B lymphocytes. Using the J-strain of Xenopus and the anticlass II monoclonal antibody, 14A2, we have studied, by indirect immunofluorescence, whether inhibition of metamorphosis would alter the pattern of expression of class II antigens during ontogeny. In control animals, class II antigens were virtually absent from thymic lymphocytes and peripheral T cells of normal untreated larvae, but could be found in increasing numbers in both populations after metamorphosis (10-12 weeks of age). In contrast, larvae, whose metamorphosis was inhibited by treatment with sodium perchlorate, had relatively few class II(+) thymic lymphocytes throughout the 6-month period of study, and the proportion of class II(+) splenic lymphocytes was approximately equal to that of IgM(+) B lymphocytes. Thus, perchlorate-treated animals retained the larval pattern of class II epression, suggesting that emergence of class II(+) T cells is dependent on metamorphosis.
format Text
id pubmed-2275827
institution National Center for Biotechnology Information
language English
publishDate 1990
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-22758272008-03-31 Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent Rollins-Smith, Louise A. Blair, Patrick Dev Immunol Research Article Class II major histocompatibility complex (MHC) antigens are expressed predominantly on B lymphocytes and macrophages of tadpoles of the South African clawed frog, Xenopus laevis, as is the pattern in lymphocyte populations of most mammals. However, unlike most mammals, young postmetamorphic frogs show expression of class II MHC antigens on a high proportion of thymocytes and most peripheral T and B lymphocytes. Using the J-strain of Xenopus and the anticlass II monoclonal antibody, 14A2, we have studied, by indirect immunofluorescence, whether inhibition of metamorphosis would alter the pattern of expression of class II antigens during ontogeny. In control animals, class II antigens were virtually absent from thymic lymphocytes and peripheral T cells of normal untreated larvae, but could be found in increasing numbers in both populations after metamorphosis (10-12 weeks of age). In contrast, larvae, whose metamorphosis was inhibited by treatment with sodium perchlorate, had relatively few class II(+) thymic lymphocytes throughout the 6-month period of study, and the proportion of class II(+) splenic lymphocytes was approximately equal to that of IgM(+) B lymphocytes. Thus, perchlorate-treated animals retained the larval pattern of class II epression, suggesting that emergence of class II(+) T cells is dependent on metamorphosis. Hindawi Publishing Corporation 1990 /pmc/articles/PMC2275827/ /pubmed/1967017 http://dx.doi.org/10.1155/1990/25197 Text en Copyright © 1990 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rollins-Smith, Louise A.
Blair, Patrick
Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent
title Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent
title_full Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent
title_fullStr Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent
title_full_unstemmed Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent
title_short Expression of Class II Major Histocompatibility Complex Antigens on Adult T Cells in Xenopus is Metamorphosis- Dependent
title_sort expression of class ii major histocompatibility complex antigens on adult t cells in xenopus is metamorphosis- dependent
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275827/
https://www.ncbi.nlm.nih.gov/pubmed/1967017
http://dx.doi.org/10.1155/1990/25197
work_keys_str_mv AT rollinssmithlouisea expressionofclassiimajorhistocompatibilitycomplexantigensonadulttcellsinxenopusismetamorphosisdependent
AT blairpatrick expressionofclassiimajorhistocompatibilitycomplexantigensonadulttcellsinxenopusismetamorphosisdependent