Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci

Twenty-eight heavy-chain variable (V(H)1) region genes and the immunoglobulin (IgM) heavy-chain constant region of an isogenic Xenopus hybrid, X. laevis/X, gilli, LG7, have been sequenced. The LG7 clone represents the first Xenopus hybrid that is homozygous for the IgH locus. The V(H)1 family was sp...

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Detalles Bibliográficos
Autores principales: Wilson, Melanie, Marcuz, Anne, Courtet, Michéle, Du Pasquier, Louis
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275904/
https://www.ncbi.nlm.nih.gov/pubmed/1343101
http://dx.doi.org/10.1155/1992/74187
Descripción
Sumario:Twenty-eight heavy-chain variable (V(H)1) region genes and the immunoglobulin (IgM) heavy-chain constant region of an isogenic Xenopus hybrid, X. laevis/X, gilli, LG7, have been sequenced. The LG7 clone represents the first Xenopus hybrid that is homozygous for the IgH locus. The V(H)1 family was specifically investigated because V(H)1 genes are used by the antibodies produced during the Xenopus antidinitrophenol (DNP) response, These V(H)1 germ-line sequences establish a so-called ”dictionary— that is available for studying somatic hypermutational mechanisms in immunized frogs.

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