Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci

Twenty-eight heavy-chain variable (V(H)1) region genes and the immunoglobulin (IgM) heavy-chain constant region of an isogenic Xenopus hybrid, X. laevis/X, gilli, LG7, have been sequenced. The LG7 clone represents the first Xenopus hybrid that is homozygous for the IgH locus. The V(H)1 family was sp...

Descripción completa

Detalles Bibliográficos
Autores principales: Wilson, Melanie, Marcuz, Anne, Courtet, Michéle, Du Pasquier, Louis
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1992
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275904/
https://www.ncbi.nlm.nih.gov/pubmed/1343101
http://dx.doi.org/10.1155/1992/74187
_version_ 1782151924245594112
author Wilson, Melanie
Marcuz, Anne
Courtet, Michéle
Du Pasquier, Louis
author_facet Wilson, Melanie
Marcuz, Anne
Courtet, Michéle
Du Pasquier, Louis
author_sort Wilson, Melanie
collection PubMed
description Twenty-eight heavy-chain variable (V(H)1) region genes and the immunoglobulin (IgM) heavy-chain constant region of an isogenic Xenopus hybrid, X. laevis/X, gilli, LG7, have been sequenced. The LG7 clone represents the first Xenopus hybrid that is homozygous for the IgH locus. The V(H)1 family was specifically investigated because V(H)1 genes are used by the antibodies produced during the Xenopus antidinitrophenol (DNP) response, These V(H)1 germ-line sequences establish a so-called ”dictionary— that is available for studying somatic hypermutational mechanisms in immunized frogs.
format Text
id pubmed-2275904
institution National Center for Biotechnology Information
language English
publishDate 1992
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-22759042008-03-31 Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci Wilson, Melanie Marcuz, Anne Courtet, Michéle Du Pasquier, Louis Dev Immunol Research Article Twenty-eight heavy-chain variable (V(H)1) region genes and the immunoglobulin (IgM) heavy-chain constant region of an isogenic Xenopus hybrid, X. laevis/X, gilli, LG7, have been sequenced. The LG7 clone represents the first Xenopus hybrid that is homozygous for the IgH locus. The V(H)1 family was specifically investigated because V(H)1 genes are used by the antibodies produced during the Xenopus antidinitrophenol (DNP) response, These V(H)1 germ-line sequences establish a so-called ”dictionary— that is available for studying somatic hypermutational mechanisms in immunized frogs. Hindawi Publishing Corporation 1992 /pmc/articles/PMC2275904/ /pubmed/1343101 http://dx.doi.org/10.1155/1992/74187 Text en Copyright © 1992 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wilson, Melanie
Marcuz, Anne
Courtet, Michéle
Du Pasquier, Louis
Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci
title Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci
title_full Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci
title_fullStr Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci
title_full_unstemmed Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci
title_short Sequences of Cμ and the V(H)1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci
title_sort sequences of cμ and the v(h)1 family in lg7, a clonable strain of xenopus, homozygous for the immunoglobulin loci
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275904/
https://www.ncbi.nlm.nih.gov/pubmed/1343101
http://dx.doi.org/10.1155/1992/74187
work_keys_str_mv AT wilsonmelanie sequencesofcmandthevh1familyinlg7aclonablestrainofxenopushomozygousfortheimmunoglobulinloci
AT marcuzanne sequencesofcmandthevh1familyinlg7aclonablestrainofxenopushomozygousfortheimmunoglobulinloci
AT courtetmichele sequencesofcmandthevh1familyinlg7aclonablestrainofxenopushomozygousfortheimmunoglobulinloci
AT dupasquierlouis sequencesofcmandthevh1familyinlg7aclonablestrainofxenopushomozygousfortheimmunoglobulinloci

Ejemplares similares