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Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen
A transgenic mouse was generated expressing on most (>80%) of thymocytes and peripheral T cells a T-cell receptor isolated from a cytotoxic T-cell clone (F5). This clone is CD8(+) and recognizes αα366-374 of the nucleoprotein (NP 366-374) of influenza virus (A/NT/60/68), in the context of Class ,...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
1993
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275926/ https://www.ncbi.nlm.nih.gov/pubmed/8281031 http://dx.doi.org/10.1155/1993/98015 |
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author | Mamalaki, Clio Elliott, James Norton, Trisha Yannoutsos, Nicholas Townsend, Alain R. Chandler, Phillip Simpson, Elizabeth Kioussis, Dimitris |
author_facet | Mamalaki, Clio Elliott, James Norton, Trisha Yannoutsos, Nicholas Townsend, Alain R. Chandler, Phillip Simpson, Elizabeth Kioussis, Dimitris |
author_sort | Mamalaki, Clio |
collection | PubMed |
description | A transgenic mouse was generated expressing on most (>80%) of thymocytes and peripheral T cells a T-cell receptor isolated from a cytotoxic T-cell clone (F5). This clone is CD8(+) and recognizes αα366-374 of the nucleoprotein (NP 366-374) of influenza virus (A/NT/60/68), in the context of Class ,MHC D(b) (Townsend et al., 1986). The receptor utilizes the Vβ11 and Vα4 gene segments for the β chain and α chain, respectively (Palmer et al., 1989). The usage of Vβ11 makes this TcR reactive to Class II IE molecules and an endogenous ligand recently identified as a product of the endogenous mammary tumour viruses (Mtv) 8, 9, and 11 (Dyson et al., 1991). Here we report the development of F5 transgenic T cells and their function in mice of the appropriate MHC (C57BL/10 H-2(b), IE(-)) or in mice expressing Class II MHC IE (e.g., CBA/Ca H-2(k) and BALB/c H-2(d)) and the endogenous Mtv ligands. Positive selection of CD8(+) T cells expressing the Vβ11 is seen in C57BL/10 transgenic mice (H-2(b)). Peripheral T cells from these mice are capable of killing target cells in an antigen-dependent manner after a period of (in vitro) culture with IL-2. In the presence of Class II MHC IE molecules and the endogenous Mtv ligand, most of the single-positive cells carrying the transgenic T-cell receptor are absent in the thymus. Unexpectedly, CD8(+) peripheral T-cells in these (H-2(k) or H-2(d)) F5 mice are predominantly Vβ11 positive and also have the capacity to kill targets in an antigen-dependent manner. This is true even following backcrossing of the F5 TcR transgene to H-2(d) scid/scid mice, in which functional rearrangement of endogenous TcR alpha- and beta-chain genes is impaired. |
format | Text |
id | pubmed-2275926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1993 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-22759262008-03-31 Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen Mamalaki, Clio Elliott, James Norton, Trisha Yannoutsos, Nicholas Townsend, Alain R. Chandler, Phillip Simpson, Elizabeth Kioussis, Dimitris Dev Immunol Research Article A transgenic mouse was generated expressing on most (>80%) of thymocytes and peripheral T cells a T-cell receptor isolated from a cytotoxic T-cell clone (F5). This clone is CD8(+) and recognizes αα366-374 of the nucleoprotein (NP 366-374) of influenza virus (A/NT/60/68), in the context of Class ,MHC D(b) (Townsend et al., 1986). The receptor utilizes the Vβ11 and Vα4 gene segments for the β chain and α chain, respectively (Palmer et al., 1989). The usage of Vβ11 makes this TcR reactive to Class II IE molecules and an endogenous ligand recently identified as a product of the endogenous mammary tumour viruses (Mtv) 8, 9, and 11 (Dyson et al., 1991). Here we report the development of F5 transgenic T cells and their function in mice of the appropriate MHC (C57BL/10 H-2(b), IE(-)) or in mice expressing Class II MHC IE (e.g., CBA/Ca H-2(k) and BALB/c H-2(d)) and the endogenous Mtv ligands. Positive selection of CD8(+) T cells expressing the Vβ11 is seen in C57BL/10 transgenic mice (H-2(b)). Peripheral T cells from these mice are capable of killing target cells in an antigen-dependent manner after a period of (in vitro) culture with IL-2. In the presence of Class II MHC IE molecules and the endogenous Mtv ligand, most of the single-positive cells carrying the transgenic T-cell receptor are absent in the thymus. Unexpectedly, CD8(+) peripheral T-cells in these (H-2(k) or H-2(d)) F5 mice are predominantly Vβ11 positive and also have the capacity to kill targets in an antigen-dependent manner. This is true even following backcrossing of the F5 TcR transgene to H-2(d) scid/scid mice, in which functional rearrangement of endogenous TcR alpha- and beta-chain genes is impaired. Hindawi Publishing Corporation 1993 /pmc/articles/PMC2275926/ /pubmed/8281031 http://dx.doi.org/10.1155/1993/98015 Text en Copyright © 1993 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mamalaki, Clio Elliott, James Norton, Trisha Yannoutsos, Nicholas Townsend, Alain R. Chandler, Phillip Simpson, Elizabeth Kioussis, Dimitris Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen |
title | Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen
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title_full | Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen
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title_fullStr | Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen
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title_full_unstemmed | Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen
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title_short | Positive and Negative Selection in Transgenic Mice Expressing a T-Cell Receptor Specific for Influenza Nucleoprotein and Endogenous Superantigen
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title_sort | positive and negative selection in transgenic mice expressing a t-cell receptor specific for influenza nucleoprotein and endogenous superantigen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275926/ https://www.ncbi.nlm.nih.gov/pubmed/8281031 http://dx.doi.org/10.1155/1993/98015 |
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