Cargando…
The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation
A rat IgM monoclonal antibody, PA3-795, inhibits the antigen-specific responses of mouse T-cell hybridomas. It recognizes a heavily glycosylated cell-surface protein, designated Lgp55, that is detectable after activation on mature T cells. During fetal life, Lgp55 is found at high levels on newly im...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
1994
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275939/ https://www.ncbi.nlm.nih.gov/pubmed/7620317 http://dx.doi.org/10.1155/1994/29157 |
_version_ | 1782151929843941376 |
---|---|
author | Mcduffie, Marcia Golde, William T. |
author_facet | Mcduffie, Marcia Golde, William T. |
author_sort | Mcduffie, Marcia |
collection | PubMed |
description | A rat IgM monoclonal antibody, PA3-795, inhibits the antigen-specific responses of mouse T-cell hybridomas. It recognizes a heavily glycosylated cell-surface protein, designated Lgp55, that is detectable after activation on mature T cells. During fetal life, Lgp55 is found at high levels on newly immigrant thymic T-cell precursors prior to surface expression of other T-lineage molecules. High levels of expression are also found on thymocytes in the outer cortex of adult mice. Thymocytes at later stages of differentiation bear decreasing amounts of surface Lgp55, and none is detectable on “single-positive” thymocytes in the thymic medulla or on resting mature T cells from the periphery. Addition of monoclonal anti-Lgp55 to fetal thymus organ culture decreases the output of “mature” CD4 singlepositive thymocytes when it is begun before fetal day 13.5. These findings suggest that Lgp55 contributes to cell-cell interactions that regulate very early steps in T-cell development in the mouse. |
format | Text |
id | pubmed-2275939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-22759392008-03-31 The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation Mcduffie, Marcia Golde, William T. Dev Immunol Research Article A rat IgM monoclonal antibody, PA3-795, inhibits the antigen-specific responses of mouse T-cell hybridomas. It recognizes a heavily glycosylated cell-surface protein, designated Lgp55, that is detectable after activation on mature T cells. During fetal life, Lgp55 is found at high levels on newly immigrant thymic T-cell precursors prior to surface expression of other T-lineage molecules. High levels of expression are also found on thymocytes in the outer cortex of adult mice. Thymocytes at later stages of differentiation bear decreasing amounts of surface Lgp55, and none is detectable on “single-positive” thymocytes in the thymic medulla or on resting mature T cells from the periphery. Addition of monoclonal anti-Lgp55 to fetal thymus organ culture decreases the output of “mature” CD4 singlepositive thymocytes when it is begun before fetal day 13.5. These findings suggest that Lgp55 contributes to cell-cell interactions that regulate very early steps in T-cell development in the mouse. Hindawi Publishing Corporation 1994 /pmc/articles/PMC2275939/ /pubmed/7620317 http://dx.doi.org/10.1155/1994/29157 Text en Copyright © 1994 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mcduffie, Marcia Golde, William T. The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation |
title | The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation |
title_full | The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation |
title_fullStr | The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation |
title_full_unstemmed | The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation |
title_short | The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation |
title_sort | accessory molecule lgp55 plays a role early in murine fetal thymocyte differentiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275939/ https://www.ncbi.nlm.nih.gov/pubmed/7620317 http://dx.doi.org/10.1155/1994/29157 |
work_keys_str_mv | AT mcduffiemarcia theaccessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation AT goldewilliamt theaccessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation AT mcduffiemarcia accessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation AT goldewilliamt accessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation |