Cargando…

The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation

A rat IgM monoclonal antibody, PA3-795, inhibits the antigen-specific responses of mouse T-cell hybridomas. It recognizes a heavily glycosylated cell-surface protein, designated Lgp55, that is detectable after activation on mature T cells. During fetal life, Lgp55 is found at high levels on newly im...

Descripción completa

Detalles Bibliográficos
Autores principales: Mcduffie, Marcia, Golde, William T.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275939/
https://www.ncbi.nlm.nih.gov/pubmed/7620317
http://dx.doi.org/10.1155/1994/29157
_version_ 1782151929843941376
author Mcduffie, Marcia
Golde, William T.
author_facet Mcduffie, Marcia
Golde, William T.
author_sort Mcduffie, Marcia
collection PubMed
description A rat IgM monoclonal antibody, PA3-795, inhibits the antigen-specific responses of mouse T-cell hybridomas. It recognizes a heavily glycosylated cell-surface protein, designated Lgp55, that is detectable after activation on mature T cells. During fetal life, Lgp55 is found at high levels on newly immigrant thymic T-cell precursors prior to surface expression of other T-lineage molecules. High levels of expression are also found on thymocytes in the outer cortex of adult mice. Thymocytes at later stages of differentiation bear decreasing amounts of surface Lgp55, and none is detectable on “single-positive” thymocytes in the thymic medulla or on resting mature T cells from the periphery. Addition of monoclonal anti-Lgp55 to fetal thymus organ culture decreases the output of “mature” CD4 singlepositive thymocytes when it is begun before fetal day 13.5. These findings suggest that Lgp55 contributes to cell-cell interactions that regulate very early steps in T-cell development in the mouse.
format Text
id pubmed-2275939
institution National Center for Biotechnology Information
language English
publishDate 1994
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-22759392008-03-31 The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation Mcduffie, Marcia Golde, William T. Dev Immunol Research Article A rat IgM monoclonal antibody, PA3-795, inhibits the antigen-specific responses of mouse T-cell hybridomas. It recognizes a heavily glycosylated cell-surface protein, designated Lgp55, that is detectable after activation on mature T cells. During fetal life, Lgp55 is found at high levels on newly immigrant thymic T-cell precursors prior to surface expression of other T-lineage molecules. High levels of expression are also found on thymocytes in the outer cortex of adult mice. Thymocytes at later stages of differentiation bear decreasing amounts of surface Lgp55, and none is detectable on “single-positive” thymocytes in the thymic medulla or on resting mature T cells from the periphery. Addition of monoclonal anti-Lgp55 to fetal thymus organ culture decreases the output of “mature” CD4 singlepositive thymocytes when it is begun before fetal day 13.5. These findings suggest that Lgp55 contributes to cell-cell interactions that regulate very early steps in T-cell development in the mouse. Hindawi Publishing Corporation 1994 /pmc/articles/PMC2275939/ /pubmed/7620317 http://dx.doi.org/10.1155/1994/29157 Text en Copyright © 1994 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mcduffie, Marcia
Golde, William T.
The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation
title The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation
title_full The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation
title_fullStr The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation
title_full_unstemmed The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation
title_short The Accessory Molecule Lgp55 Plays a Role Early in Murine Fetal Thymocyte Differentiation
title_sort accessory molecule lgp55 plays a role early in murine fetal thymocyte differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275939/
https://www.ncbi.nlm.nih.gov/pubmed/7620317
http://dx.doi.org/10.1155/1994/29157
work_keys_str_mv AT mcduffiemarcia theaccessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation
AT goldewilliamt theaccessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation
AT mcduffiemarcia accessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation
AT goldewilliamt accessorymoleculelgp55playsaroleearlyinmurinefetalthymocytedifferentiation