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A Common Stem Cell for Murine Cortical and Medullary Thymic Epithelial Cells?
We have addressed the question whether the epithelial stroma in the thymus is derived from a common stem cell or whether cortical and medullary epithelial cells are derived from different embryonic stem cells emerging, for example, from endoderm and ectoderm. By the use of rapidly expanding cultures...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275946/ https://www.ncbi.nlm.nih.gov/pubmed/9700364 http://dx.doi.org/10.1155/1995/23168 |
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author | Röpke, Carsten Van Soest, Peter Platenburg, Peter Paul Van Ewijk, Willem |
author_facet | Röpke, Carsten Van Soest, Peter Platenburg, Peter Paul Van Ewijk, Willem |
author_sort | Röpke, Carsten |
collection | PubMed |
description | We have addressed the question whether the epithelial stroma in the thymus is derived from a common stem cell or whether cortical and medullary epithelial cells are derived from different embryonic stem cells emerging, for example, from endoderm and ectoderm. By the use of rapidly expanding cultures of thymic epithelial cells (TEC) from 14 to 16 day-old murine fetuses and by specific antibodies against cortical and medullary epithelium, respectively, we were able to demonstrate a small subpopulation of double-labeled TEC in the cultures. These cells were not present in TEC cultures initiated from thymuses of neonatal mice. Double-labeled TEC were also found in tissue sections from fetal thymuses. These findings may indicate that TEC populations of the cortex and the medulla are derived from a common stem cell, with potential for differentiation toward both cortical and medullary TEC. |
format | Text |
id | pubmed-2275946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-22759462008-03-31 A Common Stem Cell for Murine Cortical and Medullary Thymic Epithelial Cells? Röpke, Carsten Van Soest, Peter Platenburg, Peter Paul Van Ewijk, Willem Dev Immunol Research Article We have addressed the question whether the epithelial stroma in the thymus is derived from a common stem cell or whether cortical and medullary epithelial cells are derived from different embryonic stem cells emerging, for example, from endoderm and ectoderm. By the use of rapidly expanding cultures of thymic epithelial cells (TEC) from 14 to 16 day-old murine fetuses and by specific antibodies against cortical and medullary epithelium, respectively, we were able to demonstrate a small subpopulation of double-labeled TEC in the cultures. These cells were not present in TEC cultures initiated from thymuses of neonatal mice. Double-labeled TEC were also found in tissue sections from fetal thymuses. These findings may indicate that TEC populations of the cortex and the medulla are derived from a common stem cell, with potential for differentiation toward both cortical and medullary TEC. Hindawi Publishing Corporation 1995 /pmc/articles/PMC2275946/ /pubmed/9700364 http://dx.doi.org/10.1155/1995/23168 Text en Copyright © 1995 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Röpke, Carsten Van Soest, Peter Platenburg, Peter Paul Van Ewijk, Willem A Common Stem Cell for Murine Cortical and Medullary Thymic Epithelial Cells? |
title | A Common Stem Cell for Murine Cortical and Medullary
Thymic Epithelial Cells? |
title_full | A Common Stem Cell for Murine Cortical and Medullary
Thymic Epithelial Cells? |
title_fullStr | A Common Stem Cell for Murine Cortical and Medullary
Thymic Epithelial Cells? |
title_full_unstemmed | A Common Stem Cell for Murine Cortical and Medullary
Thymic Epithelial Cells? |
title_short | A Common Stem Cell for Murine Cortical and Medullary
Thymic Epithelial Cells? |
title_sort | common stem cell for murine cortical and medullary
thymic epithelial cells? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275946/ https://www.ncbi.nlm.nih.gov/pubmed/9700364 http://dx.doi.org/10.1155/1995/23168 |
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