Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone

We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. This clone has the phenotype B220(+), BP-1(+) , HSA (+), CD43(+) λ5(+) , and CD25(-), and has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface MHC class II molecules,...

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Detalles Bibliográficos
Autores principales: Lombard-Platet, Suzanne, Fisher, Amanda G., Meyer, Valérie, Ceredig, Rhodri
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275953/
https://www.ncbi.nlm.nih.gov/pubmed/9700358
http://dx.doi.org/10.1155/1995/10359
Descripción
Sumario:We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. This clone has the phenotype B220(+), BP-1(+) , HSA (+), CD43(+) λ5(+) , and CD25(-), and has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface MHC class II molecules, although to a much lesser extent than the mature M12.4.1 B-cell lymphoma. G12 cells can process and present the native Hen Egg Lysozyme (HEL) to an MHC class II-restricted T-cell hybridoma. The efficiency of presentation is inferior to that obtained with M12.4.1 cells. This is the first report where a pro-B cell can serve as APC in an MHC class II-restricted presentation.

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