Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone

We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. This clone has the phenotype B220(+), BP-1(+) , HSA (+), CD43(+) λ5(+) , and CD25(-), and has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface MHC class II molecules,...

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Detalles Bibliográficos
Autores principales: Lombard-Platet, Suzanne, Fisher, Amanda G., Meyer, Valérie, Ceredig, Rhodri
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275953/
https://www.ncbi.nlm.nih.gov/pubmed/9700358
http://dx.doi.org/10.1155/1995/10359
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author Lombard-Platet, Suzanne
Fisher, Amanda G.
Meyer, Valérie
Ceredig, Rhodri
author_facet Lombard-Platet, Suzanne
Fisher, Amanda G.
Meyer, Valérie
Ceredig, Rhodri
author_sort Lombard-Platet, Suzanne
collection PubMed
description We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. This clone has the phenotype B220(+), BP-1(+) , HSA (+), CD43(+) λ5(+) , and CD25(-), and has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface MHC class II molecules, although to a much lesser extent than the mature M12.4.1 B-cell lymphoma. G12 cells can process and present the native Hen Egg Lysozyme (HEL) to an MHC class II-restricted T-cell hybridoma. The efficiency of presentation is inferior to that obtained with M12.4.1 cells. This is the first report where a pro-B cell can serve as APC in an MHC class II-restricted presentation.
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spelling pubmed-22759532008-03-31 Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone Lombard-Platet, Suzanne Fisher, Amanda G. Meyer, Valérie Ceredig, Rhodri Dev Immunol Research Article We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. This clone has the phenotype B220(+), BP-1(+) , HSA (+), CD43(+) λ5(+) , and CD25(-), and has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface MHC class II molecules, although to a much lesser extent than the mature M12.4.1 B-cell lymphoma. G12 cells can process and present the native Hen Egg Lysozyme (HEL) to an MHC class II-restricted T-cell hybridoma. The efficiency of presentation is inferior to that obtained with M12.4.1 cells. This is the first report where a pro-B cell can serve as APC in an MHC class II-restricted presentation. Hindawi Publishing Corporation 1995 /pmc/articles/PMC2275953/ /pubmed/9700358 http://dx.doi.org/10.1155/1995/10359 Text en Copyright © 1995 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lombard-Platet, Suzanne
Fisher, Amanda G.
Meyer, Valérie
Ceredig, Rhodri
Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
title Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
title_full Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
title_fullStr Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
title_full_unstemmed Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
title_short Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
title_sort expression of functional mhc class ii molecules by a mouse pro-b cell clone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275953/
https://www.ncbi.nlm.nih.gov/pubmed/9700358
http://dx.doi.org/10.1155/1995/10359
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AT meyervalerie expressionoffunctionalmhcclassiimoleculesbyamouseprobcellclone
AT ceredigrhodri expressionoffunctionalmhcclassiimoleculesbyamouseprobcellclone

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