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Two Distinct Pathways of B-Cell Development in Peyer’s Patches
The developmental biology of sheep ileal and jejunal Peyer’s patches (PP) was investigated using corticosteroids to deplete immature B lymphocytes. During a 7-day treatment with dexamethasone, ileal PP follicular (iPf)B-cell proliferation was arrested and most iPfB-cells died. This resulted in folli...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
1995
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275965/ https://www.ncbi.nlm.nih.gov/pubmed/8924762 http://dx.doi.org/10.1155/1995/46974 |
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author | Griebel, Philip J. Kugelberg, Birgit Ferrari, Giorgio |
author_facet | Griebel, Philip J. Kugelberg, Birgit Ferrari, Giorgio |
author_sort | Griebel, Philip J. |
collection | PubMed |
description | The developmental biology of sheep ileal and jejunal Peyer’s patches (PP) was investigated using corticosteroids to deplete immature B lymphocytes. During a 7-day treatment with dexamethasone, ileal PP follicular (iPf)B-cell proliferation was arrested and most iPfB-cells died. This resulted in follicular involution with the survival of mesenchymal cells. No iPfB-cell proliferation was detected in follicular remnants for 4 weeks postdexamethasone treatment, and during a subsequent 3-month period, there was limited iPfB-cell proliferation that resulted in a partial regeneration of follicles. Ileal PP involution was also associated with a severe B lymphopenia that persisted for over 14 weeks and was characterized by the survival of primarily isotype-switched and CD5(+) sIgM(+) B-cells in blood. In contrast, the size of jejunal PP follicles was reduced following dexamethasone treatment, but intrafollicular B-cell proliferation was not arrested. Furthermore, within 4 weeks, the jejunal PP follicles had recovered in size and cellularity and there was no disruption in IgA plasma-cell production. Thus, dexamethasone selectively depleted iPfB-cells and revealed that the ileal and jejunal PPs contain functionally distinct B-cell populations. The partial regeneration of the iPfB-cell population indicated that either an intrafollicular, corticosteroid-resistant B-stem cell existed or that ileal PP follicles can be repopulated by circulating B-cells. Finally, the association between ileal PP involution and the absence of circulating, CD5(-) B(-)cells confirmed that this lymphoid tissue provides an essential environment for conventional sIgM(+) B-cell development. |
format | Text |
id | pubmed-2275965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-22759652008-03-31 Two Distinct Pathways of B-Cell Development in Peyer’s Patches Griebel, Philip J. Kugelberg, Birgit Ferrari, Giorgio Dev Immunol Research Article The developmental biology of sheep ileal and jejunal Peyer’s patches (PP) was investigated using corticosteroids to deplete immature B lymphocytes. During a 7-day treatment with dexamethasone, ileal PP follicular (iPf)B-cell proliferation was arrested and most iPfB-cells died. This resulted in follicular involution with the survival of mesenchymal cells. No iPfB-cell proliferation was detected in follicular remnants for 4 weeks postdexamethasone treatment, and during a subsequent 3-month period, there was limited iPfB-cell proliferation that resulted in a partial regeneration of follicles. Ileal PP involution was also associated with a severe B lymphopenia that persisted for over 14 weeks and was characterized by the survival of primarily isotype-switched and CD5(+) sIgM(+) B-cells in blood. In contrast, the size of jejunal PP follicles was reduced following dexamethasone treatment, but intrafollicular B-cell proliferation was not arrested. Furthermore, within 4 weeks, the jejunal PP follicles had recovered in size and cellularity and there was no disruption in IgA plasma-cell production. Thus, dexamethasone selectively depleted iPfB-cells and revealed that the ileal and jejunal PPs contain functionally distinct B-cell populations. The partial regeneration of the iPfB-cell population indicated that either an intrafollicular, corticosteroid-resistant B-stem cell existed or that ileal PP follicles can be repopulated by circulating B-cells. Finally, the association between ileal PP involution and the absence of circulating, CD5(-) B(-)cells confirmed that this lymphoid tissue provides an essential environment for conventional sIgM(+) B-cell development. Hindawi Publishing Corporation 1995 /pmc/articles/PMC2275965/ /pubmed/8924762 http://dx.doi.org/10.1155/1995/46974 Text en Copyright © 1995 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Griebel, Philip J. Kugelberg, Birgit Ferrari, Giorgio Two Distinct Pathways of B-Cell Development in Peyer’s Patches |
title | Two Distinct Pathways of B-Cell Development in
Peyer’s Patches |
title_full | Two Distinct Pathways of B-Cell Development in
Peyer’s Patches |
title_fullStr | Two Distinct Pathways of B-Cell Development in
Peyer’s Patches |
title_full_unstemmed | Two Distinct Pathways of B-Cell Development in
Peyer’s Patches |
title_short | Two Distinct Pathways of B-Cell Development in
Peyer’s Patches |
title_sort | two distinct pathways of b-cell development in
peyer’s patches |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275965/ https://www.ncbi.nlm.nih.gov/pubmed/8924762 http://dx.doi.org/10.1155/1995/46974 |
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