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K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers

The mouse IgG1 monoclonal antibody (mAb) K21 recognizes a 230-kD molecule (K21-Ag) on Hassall's corpuscles in the human thymus. This mAb also stains cultured thymic epithelial cells as well as other epithelial cell lines, revealing a predominant intracellular localization. Further analysis with...

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Autores principales: Imami, Nesrina, Ladyman, Heather M., Vincents, Bjarne, Al-Tubuly, Abdulhamid, Freysdóttir, Jona, Sedibane, Moditi L., Taylor-Fishwick, David A., Foxwell, Brian M.J., Ritter, Mary A.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275999/
https://www.ncbi.nlm.nih.gov/pubmed/9716904
http://dx.doi.org/10.1155/1998/29340
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author Imami, Nesrina
Ladyman, Heather M.
Vincents, Bjarne
Al-Tubuly, Abdulhamid
Freysdóttir, Jona
Sedibane, Moditi L.
Taylor-Fishwick, David A.
Foxwell, Brian M.J.
Ritter, Mary A.
author_facet Imami, Nesrina
Ladyman, Heather M.
Vincents, Bjarne
Al-Tubuly, Abdulhamid
Freysdóttir, Jona
Sedibane, Moditi L.
Taylor-Fishwick, David A.
Foxwell, Brian M.J.
Ritter, Mary A.
author_sort Imami, Nesrina
collection PubMed
description The mouse IgG1 monoclonal antibody (mAb) K21 recognizes a 230-kD molecule (K21-Ag) on Hassall's corpuscles in the human thymus. This mAb also stains cultured thymic epithelial cells as well as other epithelial cell lines, revealing a predominant intracellular localization. Further analysis with mAb K21 on other lymphoid tissues showed that it also stains cells within the germinal centers of human tonsils, both lymphoid (B) cells and some with the appearance of follicular dendritic cells. Double immunostaining of tonsil sections shows that K21-Ag is not expressed by T cells, whereas staining with anti-CD22 and -CD23 mAb revealed some doublepositive cells. A subpopulation of the lymphoid cells express the K21-Ag much more strongly. This K21(++)/CD23(++) subpopulation of cells is localized in the apical light zone of germinal centers, suggesting that K21-Ag may be an important marker for the selected centrocytes within germinal centers and may play a role in B-cell selection and/or development of B-cell memory. Flow cytometric analysis showed that K21-Ag is expressed on the surface of a very low percentage of thymocytes, tonsillar lymphocytes, and peripheral blood mononuclear cells. Analysis of purified/separated tonsillar T and B lymphocytes showed that T cells do not express the K21-Ag; in contrast, B cells express low levels of the K21-Ag, and this together with CD23 is upregulated after mitogenic stimulation. Our data therefore raise the possibility that the K2l- Ag may play a role in B-lymphocyte activation/selection.
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spelling pubmed-22759992008-03-31 K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers Imami, Nesrina Ladyman, Heather M. Vincents, Bjarne Al-Tubuly, Abdulhamid Freysdóttir, Jona Sedibane, Moditi L. Taylor-Fishwick, David A. Foxwell, Brian M.J. Ritter, Mary A. Dev Immunol Research Article The mouse IgG1 monoclonal antibody (mAb) K21 recognizes a 230-kD molecule (K21-Ag) on Hassall's corpuscles in the human thymus. This mAb also stains cultured thymic epithelial cells as well as other epithelial cell lines, revealing a predominant intracellular localization. Further analysis with mAb K21 on other lymphoid tissues showed that it also stains cells within the germinal centers of human tonsils, both lymphoid (B) cells and some with the appearance of follicular dendritic cells. Double immunostaining of tonsil sections shows that K21-Ag is not expressed by T cells, whereas staining with anti-CD22 and -CD23 mAb revealed some doublepositive cells. A subpopulation of the lymphoid cells express the K21-Ag much more strongly. This K21(++)/CD23(++) subpopulation of cells is localized in the apical light zone of germinal centers, suggesting that K21-Ag may be an important marker for the selected centrocytes within germinal centers and may play a role in B-cell selection and/or development of B-cell memory. Flow cytometric analysis showed that K21-Ag is expressed on the surface of a very low percentage of thymocytes, tonsillar lymphocytes, and peripheral blood mononuclear cells. Analysis of purified/separated tonsillar T and B lymphocytes showed that T cells do not express the K21-Ag; in contrast, B cells express low levels of the K21-Ag, and this together with CD23 is upregulated after mitogenic stimulation. Our data therefore raise the possibility that the K2l- Ag may play a role in B-lymphocyte activation/selection. Hindawi Publishing Corporation 1998 /pmc/articles/PMC2275999/ /pubmed/9716904 http://dx.doi.org/10.1155/1998/29340 Text en Copyright © 1998 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Imami, Nesrina
Ladyman, Heather M.
Vincents, Bjarne
Al-Tubuly, Abdulhamid
Freysdóttir, Jona
Sedibane, Moditi L.
Taylor-Fishwick, David A.
Foxwell, Brian M.J.
Ritter, Mary A.
K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers
title K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers
title_full K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers
title_fullStr K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers
title_full_unstemmed K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers
title_short K21-Antigen: A Molecule Shared by the Microenvironments of the Human Thymus and Germinal Centers
title_sort k21-antigen: a molecule shared by the microenvironments of the human thymus and germinal centers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275999/
https://www.ncbi.nlm.nih.gov/pubmed/9716904
http://dx.doi.org/10.1155/1998/29340
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