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Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma

The involvement of counteractive CD8(+) T-cell subsets during tumor-specific immune responses was analyzed in a syngeneic murine plasmacytoma model. CD8(+) Tc cells against the immunogenic IL-10-producing BALB/c plasmacytoma ADJ-PC-5 can be easily induced by immunization of BALB/c mice with X-irradi...

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Detalles Bibliográficos
Autores principales: Specht, Christoph, Pauels, Hans-Gerd, Becker, Christian, Kölsch, Eckehart
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276029/
https://www.ncbi.nlm.nih.gov/pubmed/9814607
http://dx.doi.org/10.1155/1998/93545
Descripción
Sumario:The involvement of counteractive CD8(+) T-cell subsets during tumor-specific immune responses was analyzed in a syngeneic murine plasmacytoma model. CD8(+) Tc cells against the immunogenic IL-10-producing BALB/c plasmacytoma ADJ-PC-5 can be easily induced by immunization of BALB/c mice with X-irradiated ADJ-PC-5 tumor cells in vivo and in vitro. However, the failure of recipient mice to mount a protective Tc response against the tumor during early stages of a real or simulated tumor growth is not due to immunological ignorance, but depends on the induction of tumor-specific tolerance, involving a population of tumorinduced CD8(+) T cells that are able to inhibit the generation of tumor-specific Tc cells in a primary ADJ-PC-5-specific MLTC, using IFN-γ as a suppressive factor. Whereas most longterm cultivated CD8(+) ADJ-PC-5-specific Tc lines produce type-1 cytokines on stimulation, at least two of them, which were derived from a primary MLTC, display a type-2 cytokine spectrum. Furthermore, the primary in vitro Tc response against ADJ-PC-5 cells shows characteristics of a Tc2 response. The Tc response is strictly depending on tumor-derived IL-10. CD8(+) Tc cells that are induced in a primary MLTC do not produce IFN-γ, and the tumor-specific Tc response is enhanced by IL-4 but suppressed by IFN-γ or IL-12. In contrast, ADJ-PC- 5-specific CD8(+) Tc cells from immunized mice are IFN-γ producing Tc1 cells. Since the primary in vitro Tc response against the tumor is suppressed even by the smallest numbers of irradiated ADJ-PC-5-specific Tc1 cells via IFN-γ these Tc1 cells behave similar to the suppressive CD8(+) T cells that are induced during early stages of ADJ-PC-5 tumorigenesis.