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Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma

The involvement of counteractive CD8(+) T-cell subsets during tumor-specific immune responses was analyzed in a syngeneic murine plasmacytoma model. CD8(+) Tc cells against the immunogenic IL-10-producing BALB/c plasmacytoma ADJ-PC-5 can be easily induced by immunization of BALB/c mice with X-irradi...

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Detalles Bibliográficos
Autores principales: Specht, Christoph, Pauels, Hans-Gerd, Becker, Christian, Kölsch, Eckehart
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276029/
https://www.ncbi.nlm.nih.gov/pubmed/9814607
http://dx.doi.org/10.1155/1998/93545
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author Specht, Christoph
Pauels, Hans-Gerd
Becker, Christian
Kölsch, Eckehart
author_facet Specht, Christoph
Pauels, Hans-Gerd
Becker, Christian
Kölsch, Eckehart
author_sort Specht, Christoph
collection PubMed
description The involvement of counteractive CD8(+) T-cell subsets during tumor-specific immune responses was analyzed in a syngeneic murine plasmacytoma model. CD8(+) Tc cells against the immunogenic IL-10-producing BALB/c plasmacytoma ADJ-PC-5 can be easily induced by immunization of BALB/c mice with X-irradiated ADJ-PC-5 tumor cells in vivo and in vitro. However, the failure of recipient mice to mount a protective Tc response against the tumor during early stages of a real or simulated tumor growth is not due to immunological ignorance, but depends on the induction of tumor-specific tolerance, involving a population of tumorinduced CD8(+) T cells that are able to inhibit the generation of tumor-specific Tc cells in a primary ADJ-PC-5-specific MLTC, using IFN-γ as a suppressive factor. Whereas most longterm cultivated CD8(+) ADJ-PC-5-specific Tc lines produce type-1 cytokines on stimulation, at least two of them, which were derived from a primary MLTC, display a type-2 cytokine spectrum. Furthermore, the primary in vitro Tc response against ADJ-PC-5 cells shows characteristics of a Tc2 response. The Tc response is strictly depending on tumor-derived IL-10. CD8(+) Tc cells that are induced in a primary MLTC do not produce IFN-γ, and the tumor-specific Tc response is enhanced by IL-4 but suppressed by IFN-γ or IL-12. In contrast, ADJ-PC- 5-specific CD8(+) Tc cells from immunized mice are IFN-γ producing Tc1 cells. Since the primary in vitro Tc response against the tumor is suppressed even by the smallest numbers of irradiated ADJ-PC-5-specific Tc1 cells via IFN-γ these Tc1 cells behave similar to the suppressive CD8(+) T cells that are induced during early stages of ADJ-PC-5 tumorigenesis.
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spelling pubmed-22760292008-03-31 Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma Specht, Christoph Pauels, Hans-Gerd Becker, Christian Kölsch, Eckehart Dev Immunol Research Article The involvement of counteractive CD8(+) T-cell subsets during tumor-specific immune responses was analyzed in a syngeneic murine plasmacytoma model. CD8(+) Tc cells against the immunogenic IL-10-producing BALB/c plasmacytoma ADJ-PC-5 can be easily induced by immunization of BALB/c mice with X-irradiated ADJ-PC-5 tumor cells in vivo and in vitro. However, the failure of recipient mice to mount a protective Tc response against the tumor during early stages of a real or simulated tumor growth is not due to immunological ignorance, but depends on the induction of tumor-specific tolerance, involving a population of tumorinduced CD8(+) T cells that are able to inhibit the generation of tumor-specific Tc cells in a primary ADJ-PC-5-specific MLTC, using IFN-γ as a suppressive factor. Whereas most longterm cultivated CD8(+) ADJ-PC-5-specific Tc lines produce type-1 cytokines on stimulation, at least two of them, which were derived from a primary MLTC, display a type-2 cytokine spectrum. Furthermore, the primary in vitro Tc response against ADJ-PC-5 cells shows characteristics of a Tc2 response. The Tc response is strictly depending on tumor-derived IL-10. CD8(+) Tc cells that are induced in a primary MLTC do not produce IFN-γ, and the tumor-specific Tc response is enhanced by IL-4 but suppressed by IFN-γ or IL-12. In contrast, ADJ-PC- 5-specific CD8(+) Tc cells from immunized mice are IFN-γ producing Tc1 cells. Since the primary in vitro Tc response against the tumor is suppressed even by the smallest numbers of irradiated ADJ-PC-5-specific Tc1 cells via IFN-γ these Tc1 cells behave similar to the suppressive CD8(+) T cells that are induced during early stages of ADJ-PC-5 tumorigenesis. Hindawi Publishing Corporation 1998 /pmc/articles/PMC2276029/ /pubmed/9814607 http://dx.doi.org/10.1155/1998/93545 Text en Copyright © 1998 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Specht, Christoph
Pauels, Hans-Gerd
Becker, Christian
Kölsch, Eckehart
Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma
title Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma
title_full Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma
title_fullStr Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma
title_full_unstemmed Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma
title_short Differential Activation of CD8(+) Tumor-Specific Tc1 and Tc2 Cells by an IL-10-Producing Murine Plasmacytoma
title_sort differential activation of cd8(+) tumor-specific tc1 and tc2 cells by an il-10-producing murine plasmacytoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276029/
https://www.ncbi.nlm.nih.gov/pubmed/9814607
http://dx.doi.org/10.1155/1998/93545
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