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Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role

Peripheral blood lymphocytes and T-cell clones produced nanogram quantities of the chemokines RANTES, MIP-lα, MIP-lβ, MCP-l, IL-8 and GRO-α as well as the motogenic cytokine HGF. In contrast, various T-leukemia cell lines at different stages of differentiation did not produce the same chemokines/cyt...

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Autores principales: Ivanoff, Jyrki, Ivanoff, Anna, Sundqvist, Karl-Gösta
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276053/
https://www.ncbi.nlm.nih.gov/pubmed/11097202
http://dx.doi.org/10.1155/2000/28085
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author Ivanoff, Jyrki
Ivanoff, Anna
Sundqvist, Karl-Gösta
author_facet Ivanoff, Jyrki
Ivanoff, Anna
Sundqvist, Karl-Gösta
author_sort Ivanoff, Jyrki
collection PubMed
description Peripheral blood lymphocytes and T-cell clones produced nanogram quantities of the chemokines RANTES, MIP-lα, MIP-lβ, MCP-l, IL-8 and GRO-α as well as the motogenic cytokine HGF. In contrast, various T-leukemia cell lines at different stages of differentiation did not produce the same chemokines/cytokines. In order to study the possible functional importance of the poor chemokine production different T-cell lines were compared with respect to development of motile forms and migration on extracellular matrix components in the absence and presence of various chemokines. RANTES, MIP-1α, MIP-1β, IL-8, GRO-α and lymphotactin did not augment the development of motile forms including the size and appearance of the pseudopodia activity of the T-leukemia cell lines. The T-cell lines migrated spontaneously on/to fibronectin in a Boyden chamber assay system. Chemokines augmented the migration of the T-leukemia cell lines on fibronectin in the Boyden system in a chemotactic fashion with peak responses at 10 to 50 ng/ml. Thus, the production of chemokines is defective, in neoplastic T-lymphocytes. The defective chemokine production does not seem to play any major role for the basic locomotor capacity of the cells but may modulate the responsiveness to exogenous chemokines.
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spelling pubmed-22760532008-03-31 Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role Ivanoff, Jyrki Ivanoff, Anna Sundqvist, Karl-Gösta Dev Immunol Research Article Peripheral blood lymphocytes and T-cell clones produced nanogram quantities of the chemokines RANTES, MIP-lα, MIP-lβ, MCP-l, IL-8 and GRO-α as well as the motogenic cytokine HGF. In contrast, various T-leukemia cell lines at different stages of differentiation did not produce the same chemokines/cytokines. In order to study the possible functional importance of the poor chemokine production different T-cell lines were compared with respect to development of motile forms and migration on extracellular matrix components in the absence and presence of various chemokines. RANTES, MIP-1α, MIP-1β, IL-8, GRO-α and lymphotactin did not augment the development of motile forms including the size and appearance of the pseudopodia activity of the T-leukemia cell lines. The T-cell lines migrated spontaneously on/to fibronectin in a Boyden chamber assay system. Chemokines augmented the migration of the T-leukemia cell lines on fibronectin in the Boyden system in a chemotactic fashion with peak responses at 10 to 50 ng/ml. Thus, the production of chemokines is defective, in neoplastic T-lymphocytes. The defective chemokine production does not seem to play any major role for the basic locomotor capacity of the cells but may modulate the responsiveness to exogenous chemokines. Hindawi Publishing Corporation 2000 /pmc/articles/PMC2276053/ /pubmed/11097202 http://dx.doi.org/10.1155/2000/28085 Text en Copyright © 2000 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ivanoff, Jyrki
Ivanoff, Anna
Sundqvist, Karl-Gösta
Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role
title Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role
title_full Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role
title_fullStr Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role
title_full_unstemmed Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role
title_short Defective Chemokine Production in T-Leukemia Cell Lines and its Possible Functional Role
title_sort defective chemokine production in t-leukemia cell lines and its possible functional role
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276053/
https://www.ncbi.nlm.nih.gov/pubmed/11097202
http://dx.doi.org/10.1155/2000/28085
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