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Close Vicinity of PrP Expressing Cells (FDC) with Noradrenergic Fibers in Healthy Sheep Spleen
In naturally and experimentally occurring scrapie in sheep, prions invade the immune system and replicate in lymphoid organs. Here we analysed immunohistochemically, in seven spleens of 6-month-old healthy sheep, the nature of the cells expressing prion protein (PrP) potentially supporting prion rep...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276079/ https://www.ncbi.nlm.nih.gov/pubmed/11785673 http://dx.doi.org/10.1155/2001/40871 |
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author | Bencsik, A. Lezmi, S. Hunsmann, G. Baron, T. |
author_facet | Bencsik, A. Lezmi, S. Hunsmann, G. Baron, T. |
author_sort | Bencsik, A. |
collection | PubMed |
description | In naturally and experimentally occurring scrapie in sheep, prions invade the immune system and replicate in lymphoid organs. Here we analysed immunohistochemically, in seven spleens of 6-month-old healthy sheep, the nature of the cells expressing prion protein (PrP) potentially supporting prion replication, as well as their relationship with autonomic innervation. PrP was identified using either RB1 rabbit antiserum or 4F2 monoclonal antibody directed against AA 108–123 portion of the bovine and AA 79–92 of human prion protein respectively. Using double labelling analysis, we demonstrated that PrPc is expressed by follicular dendritic cells using a specific monoclonal antibody (CNA42). We also showed the close vicinity of these PrP expressing cells with noradrenergic fibers, using a polyclonal tyrosine hydroxylase antibody. Our results may help the study of the cellular requirements for the possible neuroinvasion from the spleen. |
format | Text |
id | pubmed-2276079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-22760792008-03-31 Close Vicinity of PrP Expressing Cells (FDC) with Noradrenergic Fibers in Healthy Sheep Spleen Bencsik, A. Lezmi, S. Hunsmann, G. Baron, T. Dev Immunol Research Article In naturally and experimentally occurring scrapie in sheep, prions invade the immune system and replicate in lymphoid organs. Here we analysed immunohistochemically, in seven spleens of 6-month-old healthy sheep, the nature of the cells expressing prion protein (PrP) potentially supporting prion replication, as well as their relationship with autonomic innervation. PrP was identified using either RB1 rabbit antiserum or 4F2 monoclonal antibody directed against AA 108–123 portion of the bovine and AA 79–92 of human prion protein respectively. Using double labelling analysis, we demonstrated that PrPc is expressed by follicular dendritic cells using a specific monoclonal antibody (CNA42). We also showed the close vicinity of these PrP expressing cells with noradrenergic fibers, using a polyclonal tyrosine hydroxylase antibody. Our results may help the study of the cellular requirements for the possible neuroinvasion from the spleen. Hindawi Publishing Corporation 2001 /pmc/articles/PMC2276079/ /pubmed/11785673 http://dx.doi.org/10.1155/2001/40871 Text en Copyright © 2001 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bencsik, A. Lezmi, S. Hunsmann, G. Baron, T. Close Vicinity of PrP Expressing Cells (FDC) with Noradrenergic Fibers in Healthy Sheep Spleen |
title | Close Vicinity of PrP Expressing Cells (FDC) with
Noradrenergic Fibers in Healthy Sheep Spleen |
title_full | Close Vicinity of PrP Expressing Cells (FDC) with
Noradrenergic Fibers in Healthy Sheep Spleen |
title_fullStr | Close Vicinity of PrP Expressing Cells (FDC) with
Noradrenergic Fibers in Healthy Sheep Spleen |
title_full_unstemmed | Close Vicinity of PrP Expressing Cells (FDC) with
Noradrenergic Fibers in Healthy Sheep Spleen |
title_short | Close Vicinity of PrP Expressing Cells (FDC) with
Noradrenergic Fibers in Healthy Sheep Spleen |
title_sort | close vicinity of prp expressing cells (fdc) with
noradrenergic fibers in healthy sheep spleen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276079/ https://www.ncbi.nlm.nih.gov/pubmed/11785673 http://dx.doi.org/10.1155/2001/40871 |
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