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Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia
A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multip...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2277458/ https://www.ncbi.nlm.nih.gov/pubmed/18421377 http://dx.doi.org/10.1371/journal.ppat.1000047 |
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author | Delgado, Simon Erickson, Bobbie R. Agudo, Roberto Blair, Patrick J. Vallejo, Efrain Albariño, César G. Vargas, Jorge Comer, James A. Rollin, Pierre E. Ksiazek, Thomas G. Olson, James G. Nichol, Stuart T. |
author_facet | Delgado, Simon Erickson, Bobbie R. Agudo, Roberto Blair, Patrick J. Vallejo, Efrain Albariño, César G. Vargas, Jorge Comer, James A. Rollin, Pierre E. Ksiazek, Thomas G. Olson, James G. Nichol, Stuart T. |
author_sort | Delgado, Simon |
collection | PubMed |
description | A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. A non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by IFA staining with a rabbit polyvalent antiserum raised against South American arenaviruses known to be associated with HF (Guanarito, Machupo, and Sabiá). RT-PCR analysis and subsequent analysis of the complete virus S and L RNA segment sequences identified the virus as a member of the New World Clade B arenaviruses, which includes all the pathogenic South American arenaviruses. The virus was shown to be most closely related to Sabiá virus, but with 26% and 30% nucleotide difference in the S and L segments, and 26%, 28%, 15% and 22% amino acid differences for the L, Z, N, and GP proteins, respectively, indicating the virus represents a newly discovered arenavirus, for which we propose the name Chapare virus. In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia. |
format | Text |
id | pubmed-2277458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-22774582008-04-18 Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia Delgado, Simon Erickson, Bobbie R. Agudo, Roberto Blair, Patrick J. Vallejo, Efrain Albariño, César G. Vargas, Jorge Comer, James A. Rollin, Pierre E. Ksiazek, Thomas G. Olson, James G. Nichol, Stuart T. PLoS Pathog Research Article A small focus of hemorrhagic fever (HF) cases occurred near Cochabamba, Bolivia, in December 2003 and January 2004. Specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. A non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by IFA staining with a rabbit polyvalent antiserum raised against South American arenaviruses known to be associated with HF (Guanarito, Machupo, and Sabiá). RT-PCR analysis and subsequent analysis of the complete virus S and L RNA segment sequences identified the virus as a member of the New World Clade B arenaviruses, which includes all the pathogenic South American arenaviruses. The virus was shown to be most closely related to Sabiá virus, but with 26% and 30% nucleotide difference in the S and L segments, and 26%, 28%, 15% and 22% amino acid differences for the L, Z, N, and GP proteins, respectively, indicating the virus represents a newly discovered arenavirus, for which we propose the name Chapare virus. In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia. Public Library of Science 2008-04-18 /pmc/articles/PMC2277458/ /pubmed/18421377 http://dx.doi.org/10.1371/journal.ppat.1000047 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Delgado, Simon Erickson, Bobbie R. Agudo, Roberto Blair, Patrick J. Vallejo, Efrain Albariño, César G. Vargas, Jorge Comer, James A. Rollin, Pierre E. Ksiazek, Thomas G. Olson, James G. Nichol, Stuart T. Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia |
title | Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia |
title_full | Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia |
title_fullStr | Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia |
title_full_unstemmed | Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia |
title_short | Chapare Virus, a Newly Discovered Arenavirus Isolated from a Fatal Hemorrhagic Fever Case in Bolivia |
title_sort | chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in bolivia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2277458/ https://www.ncbi.nlm.nih.gov/pubmed/18421377 http://dx.doi.org/10.1371/journal.ppat.1000047 |
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