Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit

Myt1 was originally identified as an inhibitory kinase for Cdc2 (Cdk1), the master engine of mitosis, and has been thought to function, together with Wee1, as a negative regulator of mitotic entry. In this study, we report an unexpected finding that Myt1 is essential for Golgi and endoplasmic reticu...

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Autores principales: Nakajima, Hiroyuki, Yonemura, Shigenobu, Murata, Masayuki, Nakamura, Nobuhiro, Piwnica-Worms, Helen, Nishida, Eisuke
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2287290/
https://www.ncbi.nlm.nih.gov/pubmed/18378775
http://dx.doi.org/10.1083/jcb.200708176
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author Nakajima, Hiroyuki
Yonemura, Shigenobu
Murata, Masayuki
Nakamura, Nobuhiro
Piwnica-Worms, Helen
Nishida, Eisuke
author_facet Nakajima, Hiroyuki
Yonemura, Shigenobu
Murata, Masayuki
Nakamura, Nobuhiro
Piwnica-Worms, Helen
Nishida, Eisuke
author_sort Nakajima, Hiroyuki
collection PubMed
description Myt1 was originally identified as an inhibitory kinase for Cdc2 (Cdk1), the master engine of mitosis, and has been thought to function, together with Wee1, as a negative regulator of mitotic entry. In this study, we report an unexpected finding that Myt1 is essential for Golgi and endoplasmic reticulum (ER) assembly during telophase in mammalian cells. Our analyses reveal that both cyclin B1 and cyclin B2 serve as targets of Myt1 for proper Golgi and ER assembly to occur. Thus, our results show that Myt1-mediated suppression of Cdc2 activity is not indispensable for the regulation of a broad range of mitotic events but is specifically required for the control of intracellular membrane dynamics during mitosis.
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spelling pubmed-22872902008-10-07 Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit Nakajima, Hiroyuki Yonemura, Shigenobu Murata, Masayuki Nakamura, Nobuhiro Piwnica-Worms, Helen Nishida, Eisuke J Cell Biol Research Articles Myt1 was originally identified as an inhibitory kinase for Cdc2 (Cdk1), the master engine of mitosis, and has been thought to function, together with Wee1, as a negative regulator of mitotic entry. In this study, we report an unexpected finding that Myt1 is essential for Golgi and endoplasmic reticulum (ER) assembly during telophase in mammalian cells. Our analyses reveal that both cyclin B1 and cyclin B2 serve as targets of Myt1 for proper Golgi and ER assembly to occur. Thus, our results show that Myt1-mediated suppression of Cdc2 activity is not indispensable for the regulation of a broad range of mitotic events but is specifically required for the control of intracellular membrane dynamics during mitosis. The Rockefeller University Press 2008-04-07 /pmc/articles/PMC2287290/ /pubmed/18378775 http://dx.doi.org/10.1083/jcb.200708176 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Nakajima, Hiroyuki
Yonemura, Shigenobu
Murata, Masayuki
Nakamura, Nobuhiro
Piwnica-Worms, Helen
Nishida, Eisuke
Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
title Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
title_full Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
title_fullStr Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
title_full_unstemmed Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
title_short Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit
title_sort myt1 protein kinase is essential for golgi and er assembly during mitotic exit
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2287290/
https://www.ncbi.nlm.nih.gov/pubmed/18378775
http://dx.doi.org/10.1083/jcb.200708176
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