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Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats
BACKGROUND: Influenza virus infection or vaccination evokes an antibody response to viral hemagglutinin (HA) and neuraminidase (NA) surface glycoproteins, which results in immunity against influenza A viruses of the same HA and NA subtype. A heterosubtypic immune response that offers some protection...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288601/ https://www.ncbi.nlm.nih.gov/pubmed/18355405 http://dx.doi.org/10.1186/1743-422X-5-44 |
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author | Straight, Timothy M Ottolini, Martin G Prince, Gregory A Eichelberger, Maryna C |
author_facet | Straight, Timothy M Ottolini, Martin G Prince, Gregory A Eichelberger, Maryna C |
author_sort | Straight, Timothy M |
collection | PubMed |
description | BACKGROUND: Influenza virus infection or vaccination evokes an antibody response to viral hemagglutinin (HA) and neuraminidase (NA) surface glycoproteins, which results in immunity against influenza A viruses of the same HA and NA subtype. A heterosubtypic immune response that offers some protection against different influenza A subtypes has been suggested from epidemiologic studies in human influenza outbreaks, and has been induced in experimental animal models. Original studies of such cross-protection showed that cytotoxic T lymphocytes (CTL) protect H3N2-immune mice from a lethal H1N1 infection. More recent studies in mice demonstrate that antibodies also contribute to heterosubtypic immunity (HSI). We previously demonstrated that HSI in cotton rats (Sigmodon hispidus) is characterized by protection of H3N2-immune animals from influenza H1N1-induced increase in respiratory rate (tachypnea). Alternatively, H1N1-immune animals are protected from H3N2-induced tachypnea. The experiments described in this report were designed to elucidate the immune mechanism that prevents this very early sign of disease. RESULTS: Our results show that cotton rats provided with H1N1-immune serum prior to challenge with an H3N2 virus were protected from influenza-associated tachypnea, with the degree of protection correlating with the antibody titer transferred. Immunization with an inactivated preparation of virus delivered intramuscularly also provided some protection suggesting that CTL and/or mucosal antibody responses are not required for protection. Antibodies specific for conserved epitopes present on the virus exterior are likely to facilitate this protection since prophylactic treatment of cotton rats with anti-M2e (the extracellular domain of M2) but not anti-nucleoprotein (NP) reduced virus-induced tachypnea. CONCLUSION: In the cotton rat model of heterosubtypic immunity, humoral immunity plays a role in protecting animals from influenza-induced tachypea. Partial protection against respiratory disease caused by different influenza A subtypes can be attained with either live virus administered intranasally or inactivated virus delivered intramuscularly suggesting that either vaccine regimen may provide some protection against potential pandemic outbreaks in humans. |
format | Text |
id | pubmed-2288601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22886012008-04-05 Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats Straight, Timothy M Ottolini, Martin G Prince, Gregory A Eichelberger, Maryna C Virol J Research BACKGROUND: Influenza virus infection or vaccination evokes an antibody response to viral hemagglutinin (HA) and neuraminidase (NA) surface glycoproteins, which results in immunity against influenza A viruses of the same HA and NA subtype. A heterosubtypic immune response that offers some protection against different influenza A subtypes has been suggested from epidemiologic studies in human influenza outbreaks, and has been induced in experimental animal models. Original studies of such cross-protection showed that cytotoxic T lymphocytes (CTL) protect H3N2-immune mice from a lethal H1N1 infection. More recent studies in mice demonstrate that antibodies also contribute to heterosubtypic immunity (HSI). We previously demonstrated that HSI in cotton rats (Sigmodon hispidus) is characterized by protection of H3N2-immune animals from influenza H1N1-induced increase in respiratory rate (tachypnea). Alternatively, H1N1-immune animals are protected from H3N2-induced tachypnea. The experiments described in this report were designed to elucidate the immune mechanism that prevents this very early sign of disease. RESULTS: Our results show that cotton rats provided with H1N1-immune serum prior to challenge with an H3N2 virus were protected from influenza-associated tachypnea, with the degree of protection correlating with the antibody titer transferred. Immunization with an inactivated preparation of virus delivered intramuscularly also provided some protection suggesting that CTL and/or mucosal antibody responses are not required for protection. Antibodies specific for conserved epitopes present on the virus exterior are likely to facilitate this protection since prophylactic treatment of cotton rats with anti-M2e (the extracellular domain of M2) but not anti-nucleoprotein (NP) reduced virus-induced tachypnea. CONCLUSION: In the cotton rat model of heterosubtypic immunity, humoral immunity plays a role in protecting animals from influenza-induced tachypea. Partial protection against respiratory disease caused by different influenza A subtypes can be attained with either live virus administered intranasally or inactivated virus delivered intramuscularly suggesting that either vaccine regimen may provide some protection against potential pandemic outbreaks in humans. BioMed Central 2008-03-20 /pmc/articles/PMC2288601/ /pubmed/18355405 http://dx.doi.org/10.1186/1743-422X-5-44 Text en Copyright © 2008 Straight et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Straight, Timothy M Ottolini, Martin G Prince, Gregory A Eichelberger, Maryna C Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats |
title | Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats |
title_full | Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats |
title_fullStr | Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats |
title_full_unstemmed | Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats |
title_short | Antibody contributes to heterosubtypic protection against influenza A-induced tachypnea in cotton rats |
title_sort | antibody contributes to heterosubtypic protection against influenza a-induced tachypnea in cotton rats |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288601/ https://www.ncbi.nlm.nih.gov/pubmed/18355405 http://dx.doi.org/10.1186/1743-422X-5-44 |
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