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Independent Positioning and Action of Escherichia coli Replisomes in Live Cells

A prevalent view of DNA replication has been that it is carried out in fixed “replication factories.” By tracking the progression of sister replication forks with respect to genetic loci in live Escherichia coli, we show that at initiation replisomes assemble at replication origins irrespective of w...

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Autores principales: Reyes-Lamothe, Rodrigo, Possoz, Christophe, Danilova, Olessia, Sherratt, David J.
Formato: Texto
Lenguaje:English
Publicado: Cell Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288635/
https://www.ncbi.nlm.nih.gov/pubmed/18394992
http://dx.doi.org/10.1016/j.cell.2008.01.044
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author Reyes-Lamothe, Rodrigo
Possoz, Christophe
Danilova, Olessia
Sherratt, David J.
author_facet Reyes-Lamothe, Rodrigo
Possoz, Christophe
Danilova, Olessia
Sherratt, David J.
author_sort Reyes-Lamothe, Rodrigo
collection PubMed
description A prevalent view of DNA replication has been that it is carried out in fixed “replication factories.” By tracking the progression of sister replication forks with respect to genetic loci in live Escherichia coli, we show that at initiation replisomes assemble at replication origins irrespective of where the origins are positioned within the cell. Sister replisomes separate and move to opposite cell halves shortly after initiation, migrating outwards as replication proceeds and both returning to midcell as replication termination approaches. DNA polymerase is maintained at stalled replication forks, and over short intervals of time replisomes are more dynamic than genetic loci. The data are inconsistent with models in which replisomes associated with sister forks act within a fixed replication factory. We conclude that independent replication forks follow the path of the compacted chromosomal DNA, with no structure other than DNA anchoring the replisome to any particular cellular region.
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spelling pubmed-22886352008-04-08 Independent Positioning and Action of Escherichia coli Replisomes in Live Cells Reyes-Lamothe, Rodrigo Possoz, Christophe Danilova, Olessia Sherratt, David J. Cell Article A prevalent view of DNA replication has been that it is carried out in fixed “replication factories.” By tracking the progression of sister replication forks with respect to genetic loci in live Escherichia coli, we show that at initiation replisomes assemble at replication origins irrespective of where the origins are positioned within the cell. Sister replisomes separate and move to opposite cell halves shortly after initiation, migrating outwards as replication proceeds and both returning to midcell as replication termination approaches. DNA polymerase is maintained at stalled replication forks, and over short intervals of time replisomes are more dynamic than genetic loci. The data are inconsistent with models in which replisomes associated with sister forks act within a fixed replication factory. We conclude that independent replication forks follow the path of the compacted chromosomal DNA, with no structure other than DNA anchoring the replisome to any particular cellular region. Cell Press 2008-04-04 /pmc/articles/PMC2288635/ /pubmed/18394992 http://dx.doi.org/10.1016/j.cell.2008.01.044 Text en © 2008 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Reyes-Lamothe, Rodrigo
Possoz, Christophe
Danilova, Olessia
Sherratt, David J.
Independent Positioning and Action of Escherichia coli Replisomes in Live Cells
title Independent Positioning and Action of Escherichia coli Replisomes in Live Cells
title_full Independent Positioning and Action of Escherichia coli Replisomes in Live Cells
title_fullStr Independent Positioning and Action of Escherichia coli Replisomes in Live Cells
title_full_unstemmed Independent Positioning and Action of Escherichia coli Replisomes in Live Cells
title_short Independent Positioning and Action of Escherichia coli Replisomes in Live Cells
title_sort independent positioning and action of escherichia coli replisomes in live cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288635/
https://www.ncbi.nlm.nih.gov/pubmed/18394992
http://dx.doi.org/10.1016/j.cell.2008.01.044
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