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Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development

Myosin isoforms contribute to the heterogeneity and adaptability of skeletal muscle fibers. Besides the well-characterized slow and fast muscle myosins, there are those isoforms that appear transiently during the course of muscle development. At a stage of development when two different myosins are...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1991
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288945/
https://www.ncbi.nlm.nih.gov/pubmed/1707054
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description Myosin isoforms contribute to the heterogeneity and adaptability of skeletal muscle fibers. Besides the well-characterized slow and fast muscle myosins, there are those isoforms that appear transiently during the course of muscle development. At a stage of development when two different myosins are coexpressed, the possibility arises for the existence of heterodimers, molecules containing two different heavy chains, or homodimers, molecules with two identical heavy chains. The question of whether neonatal and adult myosin isoforms can associate to form a stable heterodimer was addressed by using stage-specific monoclonal antibodies in conjunction with immunological and electron microscopic techniques. We find that independent of the ratio of adult to neonatal myosin, depending on the age of the animal, the myosin heavy chains form predominantly homodimeric molecules. The small amount of hybrid species present suggests that either the rod portion of the two heavy chain isoforms differs too much in sequence to form a stable alpha-helical coiled coil, or that the biosynthesis of the heavy chains precludes the formation of heterodimeric molecules.
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spelling pubmed-22889452008-05-01 Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development J Cell Biol Articles Myosin isoforms contribute to the heterogeneity and adaptability of skeletal muscle fibers. Besides the well-characterized slow and fast muscle myosins, there are those isoforms that appear transiently during the course of muscle development. At a stage of development when two different myosins are coexpressed, the possibility arises for the existence of heterodimers, molecules containing two different heavy chains, or homodimers, molecules with two identical heavy chains. The question of whether neonatal and adult myosin isoforms can associate to form a stable heterodimer was addressed by using stage-specific monoclonal antibodies in conjunction with immunological and electron microscopic techniques. We find that independent of the ratio of adult to neonatal myosin, depending on the age of the animal, the myosin heavy chains form predominantly homodimeric molecules. The small amount of hybrid species present suggests that either the rod portion of the two heavy chain isoforms differs too much in sequence to form a stable alpha-helical coiled coil, or that the biosynthesis of the heavy chains precludes the formation of heterodimeric molecules. The Rockefeller University Press 1991-04-02 /pmc/articles/PMC2288945/ /pubmed/1707054 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development
title Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development
title_full Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development
title_fullStr Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development
title_full_unstemmed Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development
title_short Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development
title_sort neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288945/
https://www.ncbi.nlm.nih.gov/pubmed/1707054