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Identification of sequences necessary for the association of cardiac myosin subunits
To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of s...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1991
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288968/ https://www.ncbi.nlm.nih.gov/pubmed/2016338 |
| _version_ | 1782152149158854656 |
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| collection | PubMed |
| description | To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of subfragment 1 and the amino terminus of subfragment 2 with a full-length rat cardiac myosin light chain 1. A 16 amino acid region of the myosin heavy chain (residues 792-808) was shown to be required for myosin light chain 1 binding in an immunoprecipitation assay. |
| format | Text |
| id | pubmed-2288968 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1991 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-22889682008-05-01 Identification of sequences necessary for the association of cardiac myosin subunits J Cell Biol Articles To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of subfragment 1 and the amino terminus of subfragment 2 with a full-length rat cardiac myosin light chain 1. A 16 amino acid region of the myosin heavy chain (residues 792-808) was shown to be required for myosin light chain 1 binding in an immunoprecipitation assay. The Rockefeller University Press 1991-05-01 /pmc/articles/PMC2288968/ /pubmed/2016338 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles Identification of sequences necessary for the association of cardiac myosin subunits |
| title | Identification of sequences necessary for the association of cardiac myosin subunits |
| title_full | Identification of sequences necessary for the association of cardiac myosin subunits |
| title_fullStr | Identification of sequences necessary for the association of cardiac myosin subunits |
| title_full_unstemmed | Identification of sequences necessary for the association of cardiac myosin subunits |
| title_short | Identification of sequences necessary for the association of cardiac myosin subunits |
| title_sort | identification of sequences necessary for the association of cardiac myosin subunits |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2288968/ https://www.ncbi.nlm.nih.gov/pubmed/2016338 |