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Quenched flow analysis of exocytosis in Paramecium cells: time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells
Synchronous exocytosis in Paramecium cells was analyzed on a subsecond time scale. For this purpose we developed a quenched flow device for rapid mixing and rapid freezing of cells without impairment (time resolution in the millisecond range, dead time approximately 30 ms). Cells frozen at defined t...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1991
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289032/ https://www.ncbi.nlm.nih.gov/pubmed/2045413 |