Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles

The transport of pro-alpha-factor from the ER to the Golgi apparatus in gently lysed yeast spheroplasts is mediated by diffusible vesicles. These transport vesicles contain core-glycosylated pro-alpha-factor and are physically separable from donor ER and target Golgi compartments. The formation of d...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1991
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289076/
https://www.ncbi.nlm.nih.gov/pubmed/1649197
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collection PubMed
description The transport of pro-alpha-factor from the ER to the Golgi apparatus in gently lysed yeast spheroplasts is mediated by diffusible vesicles. These transport vesicles contain core-glycosylated pro-alpha-factor and are physically separable from donor ER and target Golgi compartments. The formation of diffusible vesicles from the ER requires ATP, Sec12p, Sec23p, and GTP hydrolysis. The vesicles produced are functionally distinct from the ER: they transfer pro-alpha-factor to the Golgi apparatus faster and more efficiently than the ER, they do not require Sec12p or Sec23p to complete transfer, and transfer is resistant to GTP gamma S. Targeting of vesicles to the Golgi apparatus requires Ypt1p and Sec18p. Fusion of vesicles that have targeted requires calcium and ATP.
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spelling pubmed-22890762008-05-01 Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles J Cell Biol Articles The transport of pro-alpha-factor from the ER to the Golgi apparatus in gently lysed yeast spheroplasts is mediated by diffusible vesicles. These transport vesicles contain core-glycosylated pro-alpha-factor and are physically separable from donor ER and target Golgi compartments. The formation of diffusible vesicles from the ER requires ATP, Sec12p, Sec23p, and GTP hydrolysis. The vesicles produced are functionally distinct from the ER: they transfer pro-alpha-factor to the Golgi apparatus faster and more efficiently than the ER, they do not require Sec12p or Sec23p to complete transfer, and transfer is resistant to GTP gamma S. Targeting of vesicles to the Golgi apparatus requires Ypt1p and Sec18p. Fusion of vesicles that have targeted requires calcium and ATP. The Rockefeller University Press 1991-07-02 /pmc/articles/PMC2289076/ /pubmed/1649197 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles
title Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles
title_full Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles
title_fullStr Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles
title_full_unstemmed Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles
title_short Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles
title_sort distinct biochemical requirements for the budding, targeting, and fusion of er-derived transport vesicles
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289076/
https://www.ncbi.nlm.nih.gov/pubmed/1649197

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