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NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases

In previous work, we found that nerve growth factor (NGF) induced expression of the mRNA transcript encoding the metalloproteinase transin/stromelysin in PC12 cells. Transin was found, moreover, to be a "late" gene product whose expression correlated with neurites extension. In this study,...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1991
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289118/
https://www.ncbi.nlm.nih.gov/pubmed/1908468
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description In previous work, we found that nerve growth factor (NGF) induced expression of the mRNA transcript encoding the metalloproteinase transin/stromelysin in PC12 cells. Transin was found, moreover, to be a "late" gene product whose expression correlated with neurites extension. In this study, various aspects of the NGF intracellular signaling pathway in PC12 cells are investigated. We show that the protein kinase inhibitor staurosporine, but not various other kinase inhibitors, specifically blocked the NGF induction of transin. Preliminary characterization of this staurosporine-sensitive kinase suggest that it does not correspond to a tyrosine kinase, nor various serine kinases, and that it is involved both at the transcriptional and posttranscriptional levels of transin gene regulation. In contrast to these effects of staurosporine, various activators of protein kinases C and A augmented the NGF induction of transin. Similar effects of these kinase inhibitors and activators were also observed with the expression of various immediate-early genes that have been proposed to mediate the transcriptional effects of NGF, including c-fos and c-jun. These data suggest, therefore, that the NGF induction of transin mRNA expression involves multiple protein kinases acting at a number of postreceptor regulatory steps in the NGF signaling pathway.
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spelling pubmed-22891182008-05-01 NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases J Cell Biol Articles In previous work, we found that nerve growth factor (NGF) induced expression of the mRNA transcript encoding the metalloproteinase transin/stromelysin in PC12 cells. Transin was found, moreover, to be a "late" gene product whose expression correlated with neurites extension. In this study, various aspects of the NGF intracellular signaling pathway in PC12 cells are investigated. We show that the protein kinase inhibitor staurosporine, but not various other kinase inhibitors, specifically blocked the NGF induction of transin. Preliminary characterization of this staurosporine-sensitive kinase suggest that it does not correspond to a tyrosine kinase, nor various serine kinases, and that it is involved both at the transcriptional and posttranscriptional levels of transin gene regulation. In contrast to these effects of staurosporine, various activators of protein kinases C and A augmented the NGF induction of transin. Similar effects of these kinase inhibitors and activators were also observed with the expression of various immediate-early genes that have been proposed to mediate the transcriptional effects of NGF, including c-fos and c-jun. These data suggest, therefore, that the NGF induction of transin mRNA expression involves multiple protein kinases acting at a number of postreceptor regulatory steps in the NGF signaling pathway. The Rockefeller University Press 1991-09-01 /pmc/articles/PMC2289118/ /pubmed/1908468 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases
title NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases
title_full NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases
title_fullStr NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases
title_full_unstemmed NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases
title_short NGF-induction of the metalloproteinase-transin/stromelysin in PC12 cells: involvement of multiple protein kinases
title_sort ngf-induction of the metalloproteinase-transin/stromelysin in pc12 cells: involvement of multiple protein kinases
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289118/
https://www.ncbi.nlm.nih.gov/pubmed/1908468