Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons

High efficiency gene transfer (greater than 90%) in chicken dorsal root ganglion neurons has been obtained by DNA calcium phosphate co- precipitation, hence providing an important tool to study control of gene expression in primary neurons. Transfection with c-fos promoter sequences linked to the ch...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1992
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289524/
https://www.ncbi.nlm.nih.gov/pubmed/1618900
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collection PubMed
description High efficiency gene transfer (greater than 90%) in chicken dorsal root ganglion neurons has been obtained by DNA calcium phosphate co- precipitation, hence providing an important tool to study control of gene expression in primary neurons. Transfection with c-fos promoter sequences linked to the chloramphenicol acetyltransferase reporter gene showed that the serum responsive element functions as a strong transcriptional enhancer. Transcription from this element is developmentally regulated, and mediates the genetic response to nerve growth factor (NGF) in developing avian sensory neurons. Furthermore, NGF exerts a negative effect on transcription from the cyclic AMP responsive element, thereby supporting the involvement of tyrosine kinase activation by NGF in primary sensory neurons.
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spelling pubmed-22895242008-05-01 Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons J Cell Biol Articles High efficiency gene transfer (greater than 90%) in chicken dorsal root ganglion neurons has been obtained by DNA calcium phosphate co- precipitation, hence providing an important tool to study control of gene expression in primary neurons. Transfection with c-fos promoter sequences linked to the chloramphenicol acetyltransferase reporter gene showed that the serum responsive element functions as a strong transcriptional enhancer. Transcription from this element is developmentally regulated, and mediates the genetic response to nerve growth factor (NGF) in developing avian sensory neurons. Furthermore, NGF exerts a negative effect on transcription from the cyclic AMP responsive element, thereby supporting the involvement of tyrosine kinase activation by NGF in primary sensory neurons. The Rockefeller University Press 1992-07-01 /pmc/articles/PMC2289524/ /pubmed/1618900 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons
title Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons
title_full Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons
title_fullStr Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons
title_full_unstemmed Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons
title_short Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons
title_sort nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289524/
https://www.ncbi.nlm.nih.gov/pubmed/1618900

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