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The activity of Golgi transport vesicles depends on the presence of the N-ethylmaleimide-sensitive factor (NSF) and a soluble NSF attachment protein (alpha SNAP) during vesicle formation
An assay designed to measure the formation of functional transport vesicles was constructed by modifying a cell-free assay for protein transport between compartments of the Golgi (Balch, W. E., W. G. Dunphy, W. A. Braell, and J. E. Rothman. 1984. Cell. 39:405-416). A 35- kD cytosolic protein that is...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1992
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289609/ https://www.ncbi.nlm.nih.gov/pubmed/1522110 |