Cargando…

Localization of dystrophin COOH-terminal domain by the fracture-label technique

The precise localization of dystrophin in the skeletal muscle cell should contribute to a better understanding of the yet unclear functional role of this protein, both in normal and in Duchenne muscular dystrophy. Immunocytochemical studies did not give conclusive results on the localization of dyst...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1992
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289615/
https://www.ncbi.nlm.nih.gov/pubmed/1522114
_version_ 1782152299909480448
collection PubMed
description The precise localization of dystrophin in the skeletal muscle cell should contribute to a better understanding of the yet unclear functional role of this protein, both in normal and in Duchenne muscular dystrophy. Immunocytochemical studies did not give conclusive results on the localization of dystrophin with respect to the sarcolemma and to the cytoskeletal components. To improve the reliability of the electron microscopic immunocytochemical localization of dystrophin, a mAb against the COOH-terminus of the molecule has been used in association with the fracture-label technique, which, causing a partition of the membrane in protoplasmic and exoplasmic halves, allows a more precise dystrophin localization. The results obtained indicate that dystrophin is associated with the protoplasmic half of the plasmalemma, and the observation that it does not randomly follow the partition of the membrane is consistent with a stable association with the cytoskeleton.
format Text
id pubmed-2289615
institution National Center for Biotechnology Information
language English
publishDate 1992
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-22896152008-05-01 Localization of dystrophin COOH-terminal domain by the fracture-label technique J Cell Biol Articles The precise localization of dystrophin in the skeletal muscle cell should contribute to a better understanding of the yet unclear functional role of this protein, both in normal and in Duchenne muscular dystrophy. Immunocytochemical studies did not give conclusive results on the localization of dystrophin with respect to the sarcolemma and to the cytoskeletal components. To improve the reliability of the electron microscopic immunocytochemical localization of dystrophin, a mAb against the COOH-terminus of the molecule has been used in association with the fracture-label technique, which, causing a partition of the membrane in protoplasmic and exoplasmic halves, allows a more precise dystrophin localization. The results obtained indicate that dystrophin is associated with the protoplasmic half of the plasmalemma, and the observation that it does not randomly follow the partition of the membrane is consistent with a stable association with the cytoskeleton. The Rockefeller University Press 1992-09-02 /pmc/articles/PMC2289615/ /pubmed/1522114 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Localization of dystrophin COOH-terminal domain by the fracture-label technique
title Localization of dystrophin COOH-terminal domain by the fracture-label technique
title_full Localization of dystrophin COOH-terminal domain by the fracture-label technique
title_fullStr Localization of dystrophin COOH-terminal domain by the fracture-label technique
title_full_unstemmed Localization of dystrophin COOH-terminal domain by the fracture-label technique
title_short Localization of dystrophin COOH-terminal domain by the fracture-label technique
title_sort localization of dystrophin cooh-terminal domain by the fracture-label technique
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2289615/
https://www.ncbi.nlm.nih.gov/pubmed/1522114