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Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane

Endocytosis in yeast requires actin and clathrin. Live cell imaging has previously shown that massive actin polymerization occurs concomitant with a slow 200-nm inward movement of the endocytic coat (Kaksonen, M., Y. Sun, and D.G. Drubin. 2003. Cell. 115:475–487). However, the nature of the primary...

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Autores principales: Idrissi, Fatima-Zahra, Grötsch, Helga, Fernández-Golbano, Isabel M., Presciatto-Baschong, Cristina, Riezman, Howard, Geli, María-Isabel
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290847/
https://www.ncbi.nlm.nih.gov/pubmed/18347067
http://dx.doi.org/10.1083/jcb.200708060
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author Idrissi, Fatima-Zahra
Grötsch, Helga
Fernández-Golbano, Isabel M.
Presciatto-Baschong, Cristina
Riezman, Howard
Geli, María-Isabel
author_facet Idrissi, Fatima-Zahra
Grötsch, Helga
Fernández-Golbano, Isabel M.
Presciatto-Baschong, Cristina
Riezman, Howard
Geli, María-Isabel
author_sort Idrissi, Fatima-Zahra
collection PubMed
description Endocytosis in yeast requires actin and clathrin. Live cell imaging has previously shown that massive actin polymerization occurs concomitant with a slow 200-nm inward movement of the endocytic coat (Kaksonen, M., Y. Sun, and D.G. Drubin. 2003. Cell. 115:475–487). However, the nature of the primary endocytic profile in yeast and how clathrin and actin cooperate to generate an endocytic vesicle is unknown. In this study, we analyze the distribution of nine different proteins involved in endocytic uptake along plasma membrane invaginations using immunoelectron microscopy. We find that the primary endocytic profiles are tubular invaginations of up to 50 nm in diameter and 180 nm in length, which accumulate the endocytic coat components at the tip. Interestingly, significant actin labeling is only observed on invaginations longer than 50 nm, suggesting that initial membrane bending occurs before initiation of the slow inward movement. We also find that in the longest profiles, actin and the myosin-I Myo5p form two distinct structures that might be implicated in vesicle fission.
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spelling pubmed-22908472008-09-24 Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane Idrissi, Fatima-Zahra Grötsch, Helga Fernández-Golbano, Isabel M. Presciatto-Baschong, Cristina Riezman, Howard Geli, María-Isabel J Cell Biol Research Articles Endocytosis in yeast requires actin and clathrin. Live cell imaging has previously shown that massive actin polymerization occurs concomitant with a slow 200-nm inward movement of the endocytic coat (Kaksonen, M., Y. Sun, and D.G. Drubin. 2003. Cell. 115:475–487). However, the nature of the primary endocytic profile in yeast and how clathrin and actin cooperate to generate an endocytic vesicle is unknown. In this study, we analyze the distribution of nine different proteins involved in endocytic uptake along plasma membrane invaginations using immunoelectron microscopy. We find that the primary endocytic profiles are tubular invaginations of up to 50 nm in diameter and 180 nm in length, which accumulate the endocytic coat components at the tip. Interestingly, significant actin labeling is only observed on invaginations longer than 50 nm, suggesting that initial membrane bending occurs before initiation of the slow inward movement. We also find that in the longest profiles, actin and the myosin-I Myo5p form two distinct structures that might be implicated in vesicle fission. The Rockefeller University Press 2008-03-24 /pmc/articles/PMC2290847/ /pubmed/18347067 http://dx.doi.org/10.1083/jcb.200708060 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Idrissi, Fatima-Zahra
Grötsch, Helga
Fernández-Golbano, Isabel M.
Presciatto-Baschong, Cristina
Riezman, Howard
Geli, María-Isabel
Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane
title Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane
title_full Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane
title_fullStr Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane
title_full_unstemmed Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane
title_short Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane
title_sort distinct acto/myosin-i structures associate with endocytic profiles at the plasma membrane
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290847/
https://www.ncbi.nlm.nih.gov/pubmed/18347067
http://dx.doi.org/10.1083/jcb.200708060
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