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Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via pa...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290848/ https://www.ncbi.nlm.nih.gov/pubmed/18362182 http://dx.doi.org/10.1083/jcb.200709134 |
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author | Nemethova, Maria Auinger, Sonja Small, J. Victor |
author_facet | Nemethova, Maria Auinger, Sonja Small, J. Victor |
author_sort | Nemethova, Maria |
collection | PubMed |
description | Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via paxillin containing focal complexes contributed their proximal part to stress fiber assembly, and filopodia that folded laterally contributed to the construction of contractile bundles parallel to the cell edge. Correlated light and electron microscopy of cells labeled for actin and fascin confirmed integration of filopodia bundles into the lamella network. Inhibition of myosin II did not subdue the waving and folding motions of filopodia or their entry into the lamella, but filopodia were not then integrated into contractile arrays. Comparable results were obtained with B16 melanoma cells. These and other findings support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction. |
format | Text |
id | pubmed-2290848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22908482008-09-24 Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella Nemethova, Maria Auinger, Sonja Small, J. Victor J Cell Biol Research Articles Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via paxillin containing focal complexes contributed their proximal part to stress fiber assembly, and filopodia that folded laterally contributed to the construction of contractile bundles parallel to the cell edge. Correlated light and electron microscopy of cells labeled for actin and fascin confirmed integration of filopodia bundles into the lamella network. Inhibition of myosin II did not subdue the waving and folding motions of filopodia or their entry into the lamella, but filopodia were not then integrated into contractile arrays. Comparable results were obtained with B16 melanoma cells. These and other findings support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction. The Rockefeller University Press 2008-03-24 /pmc/articles/PMC2290848/ /pubmed/18362182 http://dx.doi.org/10.1083/jcb.200709134 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Nemethova, Maria Auinger, Sonja Small, J. Victor Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella |
title | Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella |
title_full | Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella |
title_fullStr | Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella |
title_full_unstemmed | Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella |
title_short | Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella |
title_sort | building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290848/ https://www.ncbi.nlm.nih.gov/pubmed/18362182 http://dx.doi.org/10.1083/jcb.200709134 |
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