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Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella

Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via pa...

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Detalles Bibliográficos
Autores principales: Nemethova, Maria, Auinger, Sonja, Small, J. Victor
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290848/
https://www.ncbi.nlm.nih.gov/pubmed/18362182
http://dx.doi.org/10.1083/jcb.200709134
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author Nemethova, Maria
Auinger, Sonja
Small, J. Victor
author_facet Nemethova, Maria
Auinger, Sonja
Small, J. Victor
author_sort Nemethova, Maria
collection PubMed
description Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via paxillin containing focal complexes contributed their proximal part to stress fiber assembly, and filopodia that folded laterally contributed to the construction of contractile bundles parallel to the cell edge. Correlated light and electron microscopy of cells labeled for actin and fascin confirmed integration of filopodia bundles into the lamella network. Inhibition of myosin II did not subdue the waving and folding motions of filopodia or their entry into the lamella, but filopodia were not then integrated into contractile arrays. Comparable results were obtained with B16 melanoma cells. These and other findings support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction.
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spelling pubmed-22908482008-09-24 Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella Nemethova, Maria Auinger, Sonja Small, J. Victor J Cell Biol Research Articles Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via paxillin containing focal complexes contributed their proximal part to stress fiber assembly, and filopodia that folded laterally contributed to the construction of contractile bundles parallel to the cell edge. Correlated light and electron microscopy of cells labeled for actin and fascin confirmed integration of filopodia bundles into the lamella network. Inhibition of myosin II did not subdue the waving and folding motions of filopodia or their entry into the lamella, but filopodia were not then integrated into contractile arrays. Comparable results were obtained with B16 melanoma cells. These and other findings support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction. The Rockefeller University Press 2008-03-24 /pmc/articles/PMC2290848/ /pubmed/18362182 http://dx.doi.org/10.1083/jcb.200709134 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Nemethova, Maria
Auinger, Sonja
Small, J. Victor
Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
title Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
title_full Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
title_fullStr Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
title_full_unstemmed Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
title_short Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
title_sort building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290848/
https://www.ncbi.nlm.nih.gov/pubmed/18362182
http://dx.doi.org/10.1083/jcb.200709134
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