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Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1

Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesio...

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Autores principales: Goetz, Jacky G., Joshi, Bharat, Lajoie, Patrick, Strugnell, Scott S., Scudamore, Trevor, Kojic, Liliana D., Nabi, Ivan R.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290850/
https://www.ncbi.nlm.nih.gov/pubmed/18347068
http://dx.doi.org/10.1083/jcb.200709019
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author Goetz, Jacky G.
Joshi, Bharat
Lajoie, Patrick
Strugnell, Scott S.
Scudamore, Trevor
Kojic, Liliana D.
Nabi, Ivan R.
author_facet Goetz, Jacky G.
Joshi, Bharat
Lajoie, Patrick
Strugnell, Scott S.
Scudamore, Trevor
Kojic, Liliana D.
Nabi, Ivan R.
author_sort Goetz, Jacky G.
collection PubMed
description Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in protrusive cellular regions. Thus, transmembrane crosstalk between the galectin lattice and pY14Cav1 promotes FA turnover by stabilizing FAK within FAs defining previously unknown, interdependent roles for galectin-3 and pY14Cav1 in tumor cell migration.
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spelling pubmed-22908502008-09-24 Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1 Goetz, Jacky G. Joshi, Bharat Lajoie, Patrick Strugnell, Scott S. Scudamore, Trevor Kojic, Liliana D. Nabi, Ivan R. J Cell Biol Research Articles Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in protrusive cellular regions. Thus, transmembrane crosstalk between the galectin lattice and pY14Cav1 promotes FA turnover by stabilizing FAK within FAs defining previously unknown, interdependent roles for galectin-3 and pY14Cav1 in tumor cell migration. The Rockefeller University Press 2008-03-24 /pmc/articles/PMC2290850/ /pubmed/18347068 http://dx.doi.org/10.1083/jcb.200709019 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Goetz, Jacky G.
Joshi, Bharat
Lajoie, Patrick
Strugnell, Scott S.
Scudamore, Trevor
Kojic, Liliana D.
Nabi, Ivan R.
Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
title Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
title_full Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
title_fullStr Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
title_full_unstemmed Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
title_short Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
title_sort concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2290850/
https://www.ncbi.nlm.nih.gov/pubmed/18347068
http://dx.doi.org/10.1083/jcb.200709019
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