A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards

BACKGROUND: In real-time quantitative PCR studies using absolute plasmid DNA standards, a calibration curve is developed to estimate an unknown DNA concentration. However, potential differences in the amplification performance of plasmid DNA compared to genomic DNA standards are often ignored in cal...

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Detalles Bibliográficos
Autores principales: Sivaganesan, Mano, Seifring, Shawn, Varma, Manju, Haugland, Richard A, Shanks, Orin C
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292693/
https://www.ncbi.nlm.nih.gov/pubmed/18298858
http://dx.doi.org/10.1186/1471-2105-9-120

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