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SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells
Severe acute respiratory syndrome (SARS) is a disease characterized by diffuse alveolar damage. We isolated human alveolar type II cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS-CoV replication as evidenced by RT-PCR detection of viral subgenomic RNA...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2312501/ https://www.ncbi.nlm.nih.gov/pubmed/18022664 http://dx.doi.org/10.1016/j.virol.2007.09.045 |
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author | Mossel, Eric C. Wang, Jieru Jeffers, Scott Edeen, Karen E. Wang, Shuanglin Cosgrove, Gregory P. Funk, C. Joel Manzer, Rizwan Miura, Tanya A. Pearson, Leonard D. Holmes, Kathryn V. Mason, Robert J. |
author_facet | Mossel, Eric C. Wang, Jieru Jeffers, Scott Edeen, Karen E. Wang, Shuanglin Cosgrove, Gregory P. Funk, C. Joel Manzer, Rizwan Miura, Tanya A. Pearson, Leonard D. Holmes, Kathryn V. Mason, Robert J. |
author_sort | Mossel, Eric C. |
collection | PubMed |
description | Severe acute respiratory syndrome (SARS) is a disease characterized by diffuse alveolar damage. We isolated human alveolar type II cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS-CoV replication as evidenced by RT-PCR detection of viral subgenomic RNA and an increase in virus titer. Virus titers were maximal by 24 h and peaked at approximately 10(5) pfu/mL. Two cell types within the cultures were infected. One cell type was type II cells, which were positive for SP-A, SP-C, cytokeratin, a type II cell-specific monoclonal antibody, and Ep-CAM. The other cell type was composed of spindle-shaped cells that were positive for vimentin and collagen III and likely fibroblasts. Viral replication was not detected in type I-like cells or macrophages. Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection. |
format | Text |
id | pubmed-2312501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-23125012009-03-01 SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells Mossel, Eric C. Wang, Jieru Jeffers, Scott Edeen, Karen E. Wang, Shuanglin Cosgrove, Gregory P. Funk, C. Joel Manzer, Rizwan Miura, Tanya A. Pearson, Leonard D. Holmes, Kathryn V. Mason, Robert J. Virology Article Severe acute respiratory syndrome (SARS) is a disease characterized by diffuse alveolar damage. We isolated human alveolar type II cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS-CoV replication as evidenced by RT-PCR detection of viral subgenomic RNA and an increase in virus titer. Virus titers were maximal by 24 h and peaked at approximately 10(5) pfu/mL. Two cell types within the cultures were infected. One cell type was type II cells, which were positive for SP-A, SP-C, cytokeratin, a type II cell-specific monoclonal antibody, and Ep-CAM. The other cell type was composed of spindle-shaped cells that were positive for vimentin and collagen III and likely fibroblasts. Viral replication was not detected in type I-like cells or macrophages. Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection. Elsevier Inc. 2008-03-01 2007-11-26 /pmc/articles/PMC2312501/ /pubmed/18022664 http://dx.doi.org/10.1016/j.virol.2007.09.045 Text en Copyright © 2007 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Mossel, Eric C. Wang, Jieru Jeffers, Scott Edeen, Karen E. Wang, Shuanglin Cosgrove, Gregory P. Funk, C. Joel Manzer, Rizwan Miura, Tanya A. Pearson, Leonard D. Holmes, Kathryn V. Mason, Robert J. SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells |
title | SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells |
title_full | SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells |
title_fullStr | SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells |
title_full_unstemmed | SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells |
title_short | SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells |
title_sort | sars-cov replicates in primary human alveolar type ii cell cultures but not in type i-like cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2312501/ https://www.ncbi.nlm.nih.gov/pubmed/18022664 http://dx.doi.org/10.1016/j.virol.2007.09.045 |
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