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Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis

Reversible phosphorylation is the most common posttranslational modification used in the regulation of cellular processes. This study of phosphatases and kinases required for peroxisome biogenesis is the first genome-wide analysis of phosphorylation events controlling organelle biogenesis. We evalua...

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Autores principales: Saleem, Ramsey A., Knoblach, Barbara, Mast, Fred D., Smith, Jennifer J., Boyle, John, Dobson, C. Melissa, Long-O'Donnell, Rose, Rachubinski, Richard A., Aitchison, John D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2315675/
https://www.ncbi.nlm.nih.gov/pubmed/18426976
http://dx.doi.org/10.1083/jcb.200710009
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author Saleem, Ramsey A.
Knoblach, Barbara
Mast, Fred D.
Smith, Jennifer J.
Boyle, John
Dobson, C. Melissa
Long-O'Donnell, Rose
Rachubinski, Richard A.
Aitchison, John D.
author_facet Saleem, Ramsey A.
Knoblach, Barbara
Mast, Fred D.
Smith, Jennifer J.
Boyle, John
Dobson, C. Melissa
Long-O'Donnell, Rose
Rachubinski, Richard A.
Aitchison, John D.
author_sort Saleem, Ramsey A.
collection PubMed
description Reversible phosphorylation is the most common posttranslational modification used in the regulation of cellular processes. This study of phosphatases and kinases required for peroxisome biogenesis is the first genome-wide analysis of phosphorylation events controlling organelle biogenesis. We evaluate signaling molecule deletion strains of the yeast Saccharomyces cerevisiae for presence of a green fluorescent protein chimera of peroxisomal thiolase, formation of peroxisomes, and peroxisome functionality. We find that distinct signaling networks involving glucose-mediated gene repression, derepression, oleate-mediated induction, and peroxisome formation promote stages of the biogenesis pathway. Additionally, separate classes of signaling proteins are responsible for the regulation of peroxisome number and size. These signaling networks specify the requirements of early and late events of peroxisome biogenesis. Among the numerous signaling proteins involved, Pho85p is exceptional, with functional involvements in both gene expression and peroxisome formation. Our study represents the first global study of signaling networks regulating the biogenesis of an organelle.
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spelling pubmed-23156752008-10-21 Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis Saleem, Ramsey A. Knoblach, Barbara Mast, Fred D. Smith, Jennifer J. Boyle, John Dobson, C. Melissa Long-O'Donnell, Rose Rachubinski, Richard A. Aitchison, John D. J Cell Biol Research Articles Reversible phosphorylation is the most common posttranslational modification used in the regulation of cellular processes. This study of phosphatases and kinases required for peroxisome biogenesis is the first genome-wide analysis of phosphorylation events controlling organelle biogenesis. We evaluate signaling molecule deletion strains of the yeast Saccharomyces cerevisiae for presence of a green fluorescent protein chimera of peroxisomal thiolase, formation of peroxisomes, and peroxisome functionality. We find that distinct signaling networks involving glucose-mediated gene repression, derepression, oleate-mediated induction, and peroxisome formation promote stages of the biogenesis pathway. Additionally, separate classes of signaling proteins are responsible for the regulation of peroxisome number and size. These signaling networks specify the requirements of early and late events of peroxisome biogenesis. Among the numerous signaling proteins involved, Pho85p is exceptional, with functional involvements in both gene expression and peroxisome formation. Our study represents the first global study of signaling networks regulating the biogenesis of an organelle. The Rockefeller University Press 2008-04-21 /pmc/articles/PMC2315675/ /pubmed/18426976 http://dx.doi.org/10.1083/jcb.200710009 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Saleem, Ramsey A.
Knoblach, Barbara
Mast, Fred D.
Smith, Jennifer J.
Boyle, John
Dobson, C. Melissa
Long-O'Donnell, Rose
Rachubinski, Richard A.
Aitchison, John D.
Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
title Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
title_full Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
title_fullStr Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
title_full_unstemmed Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
title_short Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
title_sort genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2315675/
https://www.ncbi.nlm.nih.gov/pubmed/18426976
http://dx.doi.org/10.1083/jcb.200710009
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