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Proteomic analysis of the EhV-86 virion

BACKGROUND: Emiliania huxleyi virus 86 (EhV-86) is the type species of the genus Coccolithovirus within the family Phycodnaviridae. The fully sequenced 407,339 bp genome is predicted to encode 473 protein coding sequences (CDSs) and is the largest Phycodnaviridae sequenced to date. The majority of E...

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Autores principales: Allen, Michael J, Howard, Julie A, Lilley, Kathryn S, Wilson, William H
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2322966/
https://www.ncbi.nlm.nih.gov/pubmed/18346272
http://dx.doi.org/10.1186/1477-5956-6-11
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author Allen, Michael J
Howard, Julie A
Lilley, Kathryn S
Wilson, William H
author_facet Allen, Michael J
Howard, Julie A
Lilley, Kathryn S
Wilson, William H
author_sort Allen, Michael J
collection PubMed
description BACKGROUND: Emiliania huxleyi virus 86 (EhV-86) is the type species of the genus Coccolithovirus within the family Phycodnaviridae. The fully sequenced 407,339 bp genome is predicted to encode 473 protein coding sequences (CDSs) and is the largest Phycodnaviridae sequenced to date. The majority of EhV-86 CDSs exhibit no similarity to proteins in the public databases. RESULTS: Proteomic analysis by 1-DE and then LC-MS/MS determined that the virion of EhV-86 is composed of at least 28 proteins, 23 of which are predicted to be membrane proteins. Besides the major capsid protein, putative function can be assigned to 4 other components of the virion: two lectin proteins, a thioredoxin and a serine/threonine protein kinase. CONCLUSION: This study represents the first steps toward the identification of the protein components that make up the EhV-86 virion. Aside from the major capsid protein, whose function in the virion is well known and defined, the nature of the other proteins suggest roles involved with viral budding, caspase activation, signalling, anti-oxidation, virus adsorption and host range determination.
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spelling pubmed-23229662008-04-18 Proteomic analysis of the EhV-86 virion Allen, Michael J Howard, Julie A Lilley, Kathryn S Wilson, William H Proteome Sci Research BACKGROUND: Emiliania huxleyi virus 86 (EhV-86) is the type species of the genus Coccolithovirus within the family Phycodnaviridae. The fully sequenced 407,339 bp genome is predicted to encode 473 protein coding sequences (CDSs) and is the largest Phycodnaviridae sequenced to date. The majority of EhV-86 CDSs exhibit no similarity to proteins in the public databases. RESULTS: Proteomic analysis by 1-DE and then LC-MS/MS determined that the virion of EhV-86 is composed of at least 28 proteins, 23 of which are predicted to be membrane proteins. Besides the major capsid protein, putative function can be assigned to 4 other components of the virion: two lectin proteins, a thioredoxin and a serine/threonine protein kinase. CONCLUSION: This study represents the first steps toward the identification of the protein components that make up the EhV-86 virion. Aside from the major capsid protein, whose function in the virion is well known and defined, the nature of the other proteins suggest roles involved with viral budding, caspase activation, signalling, anti-oxidation, virus adsorption and host range determination. BioMed Central 2008-03-17 /pmc/articles/PMC2322966/ /pubmed/18346272 http://dx.doi.org/10.1186/1477-5956-6-11 Text en Copyright © 2008 Allen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Allen, Michael J
Howard, Julie A
Lilley, Kathryn S
Wilson, William H
Proteomic analysis of the EhV-86 virion
title Proteomic analysis of the EhV-86 virion
title_full Proteomic analysis of the EhV-86 virion
title_fullStr Proteomic analysis of the EhV-86 virion
title_full_unstemmed Proteomic analysis of the EhV-86 virion
title_short Proteomic analysis of the EhV-86 virion
title_sort proteomic analysis of the ehv-86 virion
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2322966/
https://www.ncbi.nlm.nih.gov/pubmed/18346272
http://dx.doi.org/10.1186/1477-5956-6-11
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