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Search for the genes involved in oocyte maturation and early embryo development in the hen
BACKGROUND: The initial stages of development depend on mRNA and proteins accumulated in the oocyte, and during these stages, certain genes are essential for fertilization, first cleavage and embryonic genome activation. The aim of this study was first to search for avian oocyte-specific genes using...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2322995/ https://www.ncbi.nlm.nih.gov/pubmed/18312645 http://dx.doi.org/10.1186/1471-2164-9-110 |
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author | Elis, Sebastien Batellier, Florence Couty, Isabelle Balzergue, Sandrine Martin-Magniette, Marie-Laure Monget, Philippe Blesbois, Elisabeth Govoroun, Marina S |
author_facet | Elis, Sebastien Batellier, Florence Couty, Isabelle Balzergue, Sandrine Martin-Magniette, Marie-Laure Monget, Philippe Blesbois, Elisabeth Govoroun, Marina S |
author_sort | Elis, Sebastien |
collection | PubMed |
description | BACKGROUND: The initial stages of development depend on mRNA and proteins accumulated in the oocyte, and during these stages, certain genes are essential for fertilization, first cleavage and embryonic genome activation. The aim of this study was first to search for avian oocyte-specific genes using an in silico and a microarray approaches, then to investigate the temporal and spatial dynamics of the expression of some of these genes during follicular maturation and early embryogenesis. RESULTS: The in silico approach allowed us to identify 18 chicken homologs of mouse potential oocyte genes found by digital differential display. Using the chicken Affymetrix microarray, we identified 461 genes overexpressed in granulosa cells (GCs) and 250 genes overexpressed in the germinal disc (GD) of the hen oocyte. Six genes were identified using both in silico and microarray approaches. Based on GO annotations, GC and GD genes were differentially involved in biological processes, reflecting different physiological destinations of these two cell layers. Finally we studied the spatial and temporal dynamics of the expression of 21 chicken genes. According to their expression patterns all these genes are involved in different stages of final follicular maturation and/or early embryogenesis in the chicken. Among them, 8 genes (btg4, chkmos, wee, zpA, dazL, cvh, zar1 and ktfn) were preferentially expressed in the maturing occyte and cvh, zar1 and ktfn were also highly expressed in the early embryo. CONCLUSION: We showed that in silico and Affymetrix microarray approaches were relevant and complementary in order to find new avian genes potentially involved in oocyte maturation and/or early embryo development, and allowed the discovery of new potential chicken mature oocyte and chicken granulosa cell markers for future studies. Moreover, detailed study of the expression of some of these genes revealed promising candidates for maternal effect genes in the chicken. Finally, the finding concerning the different state of rRNA compared to that of mRNA during the postovulatory period shed light on some mechanisms through which oocyte to embryo transition occurs in the hen. |
format | Text |
id | pubmed-2322995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-23229952008-04-18 Search for the genes involved in oocyte maturation and early embryo development in the hen Elis, Sebastien Batellier, Florence Couty, Isabelle Balzergue, Sandrine Martin-Magniette, Marie-Laure Monget, Philippe Blesbois, Elisabeth Govoroun, Marina S BMC Genomics Research Article BACKGROUND: The initial stages of development depend on mRNA and proteins accumulated in the oocyte, and during these stages, certain genes are essential for fertilization, first cleavage and embryonic genome activation. The aim of this study was first to search for avian oocyte-specific genes using an in silico and a microarray approaches, then to investigate the temporal and spatial dynamics of the expression of some of these genes during follicular maturation and early embryogenesis. RESULTS: The in silico approach allowed us to identify 18 chicken homologs of mouse potential oocyte genes found by digital differential display. Using the chicken Affymetrix microarray, we identified 461 genes overexpressed in granulosa cells (GCs) and 250 genes overexpressed in the germinal disc (GD) of the hen oocyte. Six genes were identified using both in silico and microarray approaches. Based on GO annotations, GC and GD genes were differentially involved in biological processes, reflecting different physiological destinations of these two cell layers. Finally we studied the spatial and temporal dynamics of the expression of 21 chicken genes. According to their expression patterns all these genes are involved in different stages of final follicular maturation and/or early embryogenesis in the chicken. Among them, 8 genes (btg4, chkmos, wee, zpA, dazL, cvh, zar1 and ktfn) were preferentially expressed in the maturing occyte and cvh, zar1 and ktfn were also highly expressed in the early embryo. CONCLUSION: We showed that in silico and Affymetrix microarray approaches were relevant and complementary in order to find new avian genes potentially involved in oocyte maturation and/or early embryo development, and allowed the discovery of new potential chicken mature oocyte and chicken granulosa cell markers for future studies. Moreover, detailed study of the expression of some of these genes revealed promising candidates for maternal effect genes in the chicken. Finally, the finding concerning the different state of rRNA compared to that of mRNA during the postovulatory period shed light on some mechanisms through which oocyte to embryo transition occurs in the hen. BioMed Central 2008-02-29 /pmc/articles/PMC2322995/ /pubmed/18312645 http://dx.doi.org/10.1186/1471-2164-9-110 Text en Copyright © 2008 Elis et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Elis, Sebastien Batellier, Florence Couty, Isabelle Balzergue, Sandrine Martin-Magniette, Marie-Laure Monget, Philippe Blesbois, Elisabeth Govoroun, Marina S Search for the genes involved in oocyte maturation and early embryo development in the hen |
title | Search for the genes involved in oocyte maturation and early embryo development in the hen |
title_full | Search for the genes involved in oocyte maturation and early embryo development in the hen |
title_fullStr | Search for the genes involved in oocyte maturation and early embryo development in the hen |
title_full_unstemmed | Search for the genes involved in oocyte maturation and early embryo development in the hen |
title_short | Search for the genes involved in oocyte maturation and early embryo development in the hen |
title_sort | search for the genes involved in oocyte maturation and early embryo development in the hen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2322995/ https://www.ncbi.nlm.nih.gov/pubmed/18312645 http://dx.doi.org/10.1186/1471-2164-9-110 |
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