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A proteome-wide protein interaction map for Campylobacter jejuni

BACKGROUND: Data from large-scale protein interaction screens for humans and model eukaryotes have been invaluable for developing systems-level models of biological processes. Despite this value, only a limited amount of interaction data is available for prokaryotes. Here we report the systematic id...

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Autores principales: Parrish, Jodi R, Yu, Jingkai, Liu, Guozhen, Hines, Julie A, Chan, Jason E, Mangiola, Bernie A, Zhang, Huamei, Pacifico, Svetlana, Fotouhi, Farshad, DiRita, Victor J, Ideker, Trey, Andrews, Phillip, Finley, Russell L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2323224/
https://www.ncbi.nlm.nih.gov/pubmed/17615063
http://dx.doi.org/10.1186/gb-2007-8-7-r130
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author Parrish, Jodi R
Yu, Jingkai
Liu, Guozhen
Hines, Julie A
Chan, Jason E
Mangiola, Bernie A
Zhang, Huamei
Pacifico, Svetlana
Fotouhi, Farshad
DiRita, Victor J
Ideker, Trey
Andrews, Phillip
Finley, Russell L
author_facet Parrish, Jodi R
Yu, Jingkai
Liu, Guozhen
Hines, Julie A
Chan, Jason E
Mangiola, Bernie A
Zhang, Huamei
Pacifico, Svetlana
Fotouhi, Farshad
DiRita, Victor J
Ideker, Trey
Andrews, Phillip
Finley, Russell L
author_sort Parrish, Jodi R
collection PubMed
description BACKGROUND: Data from large-scale protein interaction screens for humans and model eukaryotes have been invaluable for developing systems-level models of biological processes. Despite this value, only a limited amount of interaction data is available for prokaryotes. Here we report the systematic identification of protein interactions for the bacterium Campylobacter jejuni, a food-borne pathogen and a major cause of gastroenteritis worldwide. RESULTS: Using high-throughput yeast two-hybrid screens we detected and reproduced 11,687 interactions. The resulting interaction map includes 80% of the predicted C. jejuni NCTC11168 proteins and places a large number of poorly characterized proteins into networks that provide initial clues about their functions. We used the map to identify a number of conserved subnetworks by comparison to protein networks from Escherichia coli and Saccharomyces cerevisiae. We also demonstrate the value of the interactome data for mapping biological pathways by identifying the C. jejuni chemotaxis pathway. Finally, the interaction map also includes a large subnetwork of putative essential genes that may be used to identify potential new antimicrobial drug targets for C. jejuni and related organisms. CONCLUSION: The C. jejuni protein interaction map is one of the most comprehensive yet determined for a free-living organism and nearly doubles the binary interactions available for the prokaryotic kingdom. This high level of coverage facilitates pathway mapping and function prediction for a large number of C. jejuni proteins as well as orthologous proteins from other organisms. The broad coverage also facilitates cross-species comparisons for the identification of evolutionarily conserved subnetworks of protein interactions.
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spelling pubmed-23232242008-04-19 A proteome-wide protein interaction map for Campylobacter jejuni Parrish, Jodi R Yu, Jingkai Liu, Guozhen Hines, Julie A Chan, Jason E Mangiola, Bernie A Zhang, Huamei Pacifico, Svetlana Fotouhi, Farshad DiRita, Victor J Ideker, Trey Andrews, Phillip Finley, Russell L Genome Biol Research BACKGROUND: Data from large-scale protein interaction screens for humans and model eukaryotes have been invaluable for developing systems-level models of biological processes. Despite this value, only a limited amount of interaction data is available for prokaryotes. Here we report the systematic identification of protein interactions for the bacterium Campylobacter jejuni, a food-borne pathogen and a major cause of gastroenteritis worldwide. RESULTS: Using high-throughput yeast two-hybrid screens we detected and reproduced 11,687 interactions. The resulting interaction map includes 80% of the predicted C. jejuni NCTC11168 proteins and places a large number of poorly characterized proteins into networks that provide initial clues about their functions. We used the map to identify a number of conserved subnetworks by comparison to protein networks from Escherichia coli and Saccharomyces cerevisiae. We also demonstrate the value of the interactome data for mapping biological pathways by identifying the C. jejuni chemotaxis pathway. Finally, the interaction map also includes a large subnetwork of putative essential genes that may be used to identify potential new antimicrobial drug targets for C. jejuni and related organisms. CONCLUSION: The C. jejuni protein interaction map is one of the most comprehensive yet determined for a free-living organism and nearly doubles the binary interactions available for the prokaryotic kingdom. This high level of coverage facilitates pathway mapping and function prediction for a large number of C. jejuni proteins as well as orthologous proteins from other organisms. The broad coverage also facilitates cross-species comparisons for the identification of evolutionarily conserved subnetworks of protein interactions. BioMed Central 2007 2007-07-05 /pmc/articles/PMC2323224/ /pubmed/17615063 http://dx.doi.org/10.1186/gb-2007-8-7-r130 Text en Copyright © 2007 Parrish et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Parrish, Jodi R
Yu, Jingkai
Liu, Guozhen
Hines, Julie A
Chan, Jason E
Mangiola, Bernie A
Zhang, Huamei
Pacifico, Svetlana
Fotouhi, Farshad
DiRita, Victor J
Ideker, Trey
Andrews, Phillip
Finley, Russell L
A proteome-wide protein interaction map for Campylobacter jejuni
title A proteome-wide protein interaction map for Campylobacter jejuni
title_full A proteome-wide protein interaction map for Campylobacter jejuni
title_fullStr A proteome-wide protein interaction map for Campylobacter jejuni
title_full_unstemmed A proteome-wide protein interaction map for Campylobacter jejuni
title_short A proteome-wide protein interaction map for Campylobacter jejuni
title_sort proteome-wide protein interaction map for campylobacter jejuni
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2323224/
https://www.ncbi.nlm.nih.gov/pubmed/17615063
http://dx.doi.org/10.1186/gb-2007-8-7-r130
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