Reliable identification of mycobacterial species by PCR-restriction enzyme analysis (PRA)-hsp65 in a reference laboratory and elaboration of a sequence-based extended algorithm of PRA-hsp65 patterns

BACKGROUND: Identification of nontuberculous mycobacteria (NTM) based on phenotypic tests is time-consuming, labor-intensive, expensive and often provides erroneous or inconclusive results. In the molecular method referred to as PRA-hsp65, a fragment of the hsp65 gene is amplified by PCR and then an...

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Detalles Bibliográficos
Autores principales: Chimara, Erica, Ferrazoli, Lucilaine, Ueky, Suely Yoko Misuka, Martins, Maria Conceição, Durham, Alan Mitchel, Arbeit, Robert D, Leão, Sylvia Cardoso
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2323382/
https://www.ncbi.nlm.nih.gov/pubmed/18366704
http://dx.doi.org/10.1186/1471-2180-8-48

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