High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients

We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propaga...

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Detalles Bibliográficos
Autores principales: Day, Daniel, Cranfield, Charles G., Gu, Min
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2324058/
https://www.ncbi.nlm.nih.gov/pubmed/23165061
http://dx.doi.org/10.1155/IJBI/2006/93438
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author Day, Daniel
Cranfield, Charles G.
Gu, Min
author_facet Day, Daniel
Cranfield, Charles G.
Gu, Min
author_sort Day, Daniel
collection PubMed
description We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope.
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spelling pubmed-23240582008-04-22 High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients Day, Daniel Cranfield, Charles G. Gu, Min Int J Biomed Imaging Article We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope. Hindawi Publishing Corporation 2006 2006-03-07 /pmc/articles/PMC2324058/ /pubmed/23165061 http://dx.doi.org/10.1155/IJBI/2006/93438 Text en Copyright © IJBI D. Day et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Day, Daniel
Cranfield, Charles G.
Gu, Min
High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
title High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
title_full High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
title_fullStr High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
title_full_unstemmed High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
title_short High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
title_sort high-speed fluorescence imaging and intensity profiling of femtosecond-induced calcium transients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2324058/
https://www.ncbi.nlm.nih.gov/pubmed/23165061
http://dx.doi.org/10.1155/IJBI/2006/93438
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AT cranfieldcharlesg highspeedfluorescenceimagingandintensityprofilingoffemtosecondinducedcalciumtransients
AT gumin highspeedfluorescenceimagingandintensityprofilingoffemtosecondinducedcalciumtransients

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