Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescen...

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Detalles Bibliográficos
Autores principales: Schulte, Roberta, Talamas, Jessica, Doucet, Christine, Hetzer, Martin W.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2329591/
https://www.ncbi.nlm.nih.gov/pubmed/18446240
http://dx.doi.org/10.1371/journal.pone.0002061
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author Schulte, Roberta
Talamas, Jessica
Doucet, Christine
Hetzer, Martin W.
author_facet Schulte, Roberta
Talamas, Jessica
Doucet, Christine
Hetzer, Martin W.
author_sort Schulte, Roberta
collection PubMed
description We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.
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spelling pubmed-23295912008-04-30 Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions Schulte, Roberta Talamas, Jessica Doucet, Christine Hetzer, Martin W. PLoS One Research Article We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment. Public Library of Science 2008-04-30 /pmc/articles/PMC2329591/ /pubmed/18446240 http://dx.doi.org/10.1371/journal.pone.0002061 Text en Hetzer et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schulte, Roberta
Talamas, Jessica
Doucet, Christine
Hetzer, Martin W.
Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions
title Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions
title_full Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions
title_fullStr Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions
title_full_unstemmed Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions
title_short Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions
title_sort single bead affinity detection (sinbad) for the analysis of protein-protein interactions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2329591/
https://www.ncbi.nlm.nih.gov/pubmed/18446240
http://dx.doi.org/10.1371/journal.pone.0002061
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AT talamasjessica singlebeadaffinitydetectionsinbadfortheanalysisofproteinproteininteractions
AT doucetchristine singlebeadaffinitydetectionsinbadfortheanalysisofproteinproteininteractions
AT hetzermartinw singlebeadaffinitydetectionsinbadfortheanalysisofproteinproteininteractions

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